解剖学报

›› 2010, Vol. 41 ›› Issue (4): 491-497.doi: 10.3969/j.issn.0529-1356.2010.04.002

• 论著 • 上一篇    下一篇

磷脂酶C-三磷酸肌醇途径参与β-淀粉样肽（25-35）诱导的海马神经元［Ca 2+］i的增加

林智颖1 ;陈晓春1* ; 张静1 ;林楠1 ;林凌2; 林清2 ;王玮2   

  1. 1.福建医科大学附属协和医院 福建省老年医学研究所;2.神经生物学研究中心,福州 350001
  • 收稿日期:2009-10-12 修回日期:2009-11-11 出版日期:2010-08-06
  • 通讯作者: 陈晓春

Pathway of phospholipase C-inositol trisphosphate is involved in the increase of ［CaSUP>2+/SUP>］i induced by β-amyloid peptide (25-35) in hippocampal neurons

  1. 1.Fujian Institute of Geriatrics, the Affiliated Union Hospital of Fujian Medical University;2. Research Center of Neurobiology, Fujian Medical University, Fuzhou 350001, China
  • Received:2009-10-12 Revised:2009-11-11 Online:2010-08-06
  • Contact: CHEN Xiao-chun

关键词: β-淀粉样肽（25-35）, 海马神经元, 阿尔茨海默病, 内质网钙库, 激光扫描共焦显微镜, 免疫细胞化学, 大鼠

Abstract: Objective To investigate the effect of endoplasmic reticulum(ER) calcium stores on calcium overload induced by β-amyloid peptide (25-35)(AβSUB>25-35/SUB>) in hippocampal neurons. Methods The hippocampal neurons were loaded with calcium-sensitive fluorescent indicator Fluo-3/AM.Intracellular calcium concentration (［CaSUP>2+/SUP>］i)changes were measured in different conditions of drug intervention by using laser scanning confocal microscopy. Results Groups of 2, 10, 20μmol/L AβSUB>25-35/SUB> all increased ［CaSUP>2+/SUP>］i in hippocampal neurons(EM>n/EM>=5, EM>P/EM><0.05). 2-APB, an inhibitor of ER CaSUP>2+/SUP> release through channels associated to inositol trisphosphate receptor(IP3R), was shown to prevent the aggregated AβSUB>25-35/SUB>induced rise of ［CaSUP>2+/SUP>］i, suggesting the involvement of CaSUP>2+/SUP>released by ER. However, dantrolene, an inhibitor of ER CaSUP>2+/SUP> release through channels associated to ryanodine receptor(RyR), could not prevent the rise of ［CaSUP>2+/SUP>］i induced by AβSUB>25-35/SUB>. Treatment with aggregated AβSUB>25-35/SUB> for 1 hour induced a significant decrease in the ER CaSUP>2+/SUP> content (EM>P/EM><0.01), which was more pronounced 24 hours after the addition of aggregated AβSUB>

Key words: β-amyloid peptide（25-35）, Hippocampal neuron, Alzheimer disease, Endoplasmic reticulum calcium stores, Laser scanning confocal microscopy, Immunocytochemistry, Rat

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