解剖学报

›› 2010, Vol. 41 ›› Issue (6): 823-826.doi: 10.3969/j.issn.0529-1356.2010.05.009

• 论著 • 上一篇    下一篇

嗅鞘细胞移植入淀粉样前体蛋白转基因小鼠脑内降低β淀粉样蛋白沉积

曹敬丽1 ;刘婧媛1; 肖娟1; 姜晓荣1 ;任玉水1 ;王洪美1 ;周长满1，4; 黄红云1，2，3*   

  1. 1.北京市虹天济神经科学研究院，北京 100144; 2.北京康复中心神经外科，北京 100144;3.泰山医学院神经科学研究所，山东 泰安 100144; 4.北京大学基础医学院解剖学系，北京 100191
  • 收稿日期:2009-11-30 修回日期:2010-03-17 出版日期:2010-12-06
  • 通讯作者: 黄红云

Olfactory ensheathing cells transplanation reduced amyloid burden in amyloid precursor protein transgenic mouse model

  1. 1.Beijing Hongtianji Neuroscience Academy, Beijing 100144,China; 2.Department of Neurosurgery, Beijing Rehabilitation Center, Beijing 100144, China; 3.Neuroscience Institute of Taishan Medical Uniersity, Shandong Taian 271016, China;4. Department of Anatomy and Embryology, Peking University Health Science Center, Beijing 100191,China
  • Received:2009-11-30 Revised:2010-03-17 Online:2010-12-06
  • Contact: HUANG Hong-yun

关键词: 阿尔茨海默病, β淀粉样蛋白, 细胞移植, 免疫印迹法, APP转基因小鼠

Abstract: Objective To observe the survival and migration of olfactory ensheathing cells (OECs) after transplanted into amyloid precursor protein (APP) transgenic mice and investigate its therapeutical effect on Alzheimer disease. Methods OECs from olfactory ensheathing bulb of enhanced green fluorescent protein(EGFP) transgenic mice were isolated, then transplanted into an APP transgenic mice. Stereotaxic coordinates and anterior-posterior were appilicated for microinjection. Confocal microscope was used to observe the survival and migration of the cells, immunohistochemistry and Western blotting were carried out to survey amyloid burden, 4 mice in each group. Results The results showed that OECs survived well and migrated away from injection site after transplanted into APP transgenic mice, the vascular phenomena of OECs was also observed, and the therapy reduced amyloid burden of Alzheimers disease model.Quantitative test showed that APP reduced significantly（EM>P/EM><0.05）. Conclusion Olfactory ensheathing cells do some help to reduce the amyloid burden in the brain of APP transgenic mice.

Key words: Alzheimer disease, β-amyloid protein, Cell transplantation, Western blotting, Amyloid precursor protein transgenic mouse

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