关键词: 卵巢上皮癌, 永生化, 细胞系, 端着丝粒染色体, 肿瘤干细胞, 染色体分析, 人

Abstract: Objective According to the usual method to establish tumor cell line, tissue of human ovarian epithelial carcinoma was transplanted to the immunodeficient mice and a immortalized cell line was established by primary culture, so to provide one more experimental model for the investigation of human carcinoma. Methods The cancer tissue from a patient with ovarian papillary serous carcinoma IIIcG3 was transplanted subcutaneously into node mice. A suspension cell line was established by primary culture EM>in vitro/EM>. The morphology of the cell line was observed by light microscope and electro microscope. Its biologic characteristics were investigated by growth curve, karyotype analysis, soft agar culture and nude mice transplantation. Results This cell line was named WSZ.It has been maintained for over one hundred passages. It was in suspension condition with vigorous growth.The population doubling time of cells in logarithmic growth phage was about 15.8 hours. The number of chromosomes was from 16 to 135 and 68 or 69 was multiple. Karyotype analysis showed that almost all the chromosomes were telocentric. It had a clone forming rate of 89.3% and could form clones in soft agar.It was demonstrated that toumor could be formed under the skin of nude mouse with cell number of 10SUP>6/SUP> while in non-obese diabetes（NOD) serve combined immuno-deficiency(SCID) only 100 cells were needed. Conclusion A malignet cell line with telocentric chromosomes derived from nude mouse transplanted by ovarian epithelial curcinoma was established, it is named WSZ is line, that possesses typical malignent characteristics. WSZ had stable infinite proliferation ability. WSZ is a cell line with high malignant.

Key words: Ovarian epithelial carcinoma, Immortalization, Cell line, Telocentric chromosome, Cancer stem cells, Chromosome analysis, Human