解剖学报

›› 2011, Vol. 42 ›› Issue (2): 151-154.doi: 10.3969/j.issn.0529-1356.2011.02.002

• 论著 • 上一篇    下一篇

创伤后应激障碍大鼠前额叶内侧皮质糖皮质激素受体表达增高

张景华1,2 ;李慢1; 石玉秀1* ;韩芳1   

  1. 1.中国医科大学基础医学院组织学与胚胎学教研室，沈阳 110000;2.沈阳军区总医院神经内科，沈阳 110016
  • 收稿日期:2010-07-20 修回日期:2010-09-20 出版日期:2011-04-06
  • 通讯作者: 石玉秀

Expression of glucocorticoid receptors in medial prefrontal cortex is increased in posttraumatic stress disorder rats

  1. 1.Department of Histology and Embryology, College of Basic Medical Sciences, China Medical University, Shenyang110001, China;2.Department of Neurology, General Hospital of Shenyang Military Area Command of Chinese PLA，Shenyang 110016,China
  • Received:2010-07-20 Revised:2010-09-20 Online:2011-04-06
  • Contact: SHI Yu-xiu

关键词: 创伤后应激障碍, 前额叶内侧皮质, 糖皮质激素受体, 免疫组织化学, 免疫印迹法, 反转录-聚合酶链反应, 大鼠

Abstract: ObjectiveTo observe the expression of glucocorticoid receptors (GR) in the medial prefrontal cortex (mPFC) of posttraumatic stress disorder (PTSD) rats. MethodsThe single prolonged stress (SPS) method was used to set up the rat PTSD models. A total of ninety male Wistar rats were randomly divided into 1 day, 7 days, 14 days, 28 days of SPS groups and normal control group. Immunohistochemistry,Western blotting, RT-PCR and image analysis and statistical analysis were used to detect the activity of GR. ResultsThe activity of GR in mPFC neurons gradually increased at the 7th day and the 14th day after exposure to SPS compared with the control group, reached the maximum at the 14th day after exposure to SPS, the 28th day restorative down, but still higher than the control group (EM>P/EM><0.05). ConclusionExpression of GR may be related to the pathogenesis of hypothalamic-pituitary-adrenal axis.

Key words: Posttraumatic stress disorder, Medial prefrontal cortex, Glucocorticoid receptor, Immunohistochemistry, Western blotting, RT-PCR, Rat

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