›› 2011, Vol. 42 ›› Issue (2): 195-200.doi: 10.3969/j.issn.0529-1356.2011.02.011

• 论著 • 上一篇    下一篇

非小细胞肺癌患者紧密连接蛋白-1基因启动子甲基化分析及其临床意义

王晔恺;周吉航;曾芳;周世权;黄燕燕;刘晓光*   

  1. 舟山医院细胞分子生物学实验室,浙江 舟山316004
  • 收稿日期:2010-06-28 修回日期:2010-08-06 出版日期:2011-04-06
  • 通讯作者: 刘晓光

Analysis and clinical significance of methylation status of zonula occluden-1 promoter in patients with non-small cell lung cancer

  1. Cellular and Molecular Laboratory, Zhoushan Hospital, Zhejiang Zhoushan 316004,China
  • Received:2010-06-28 Revised:2010-08-06 Online:2011-04-06
  • Contact: LIU Xiao-guang

关键词: 紧密连接蛋白-1基因, 启动子, 非小细胞肺癌, 甲基化聚合酶链反应, 实时荧光定量聚合酶链反应

Abstract: Objective The objective of this study is to investigate the methylation status of zonula occluden-1 (ZO-1) gene in patients with non-small cell lung cancer (NSCLC) and to indentify this roles in pathogenesis,development and classification of NSCLC.Methods The methylation status of ZO-1 gene of 101 patients with NSCLC and 61 patients with benign lung disease were detected by methylationspecific-polymerase chain reaction (MS-PCR). ZO-1 mRNA and protein of 200 unmethylation tissue and 62 methylation tissue were detected by real-time PCR and Western blotting separately. Result Both of ZO-1 mRNA and protein was significant statistic different between methylation group and unmethylation group(EM>t/EM>=-26.028,P<0.01,EM>t/EM> =-37.216,EM>P/EM><0.01).There was significant statistic difference between carcinoma tissue group(32.7%,33/100) and control group(11.5%,7/61) (EM>χ/EM>SUP>2/SUP>=9.190,EM>P/EM><0.01). ZO-1 promoter methylation status in adjacent tissues was significant statistic different between lymph node invasion group(35.5%,11/31) and lymph nodes without invasion group(17.1%,12/70) (EM>χ/EM>SUP>2/SUP>=4.110,EM>P/EM><0.05). ZO-1 promoter methylation status of carcinoma tissues wase significant statistic different between 2-year suvival group (27.5%,14/51) and 2-year death group(43.2%,19/44) (EM>χ/EM>SUP>2/SUP>=4.150, EM>P/EM><0.05). Conclusion Methylation status of ZO-1 promoter may affect the transcription of mRNA and expression of protein. ZO-1 promoter methylation status of adjacent tissues and carcinoma tissues in N

Key words: Zonula occluden-1, Promoter, Non-small cell lung cancer, Methylation specificPCR, Real-time PCR

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