解剖学报

解剖学报 ›› 2017, Vol. 48 ›› Issue (2): 156-159.doi: 10.16098/j.issn.0529-1356.2017.02.006

• 肿瘤生物学 • 上一篇    下一篇

非小细胞肺癌放疗抵抗细胞模型的建立与鉴定

齐淑雅1 施汉飞1 石梦婕1 张孟辉1 左彦珍2* 孙大永3   

  1. 1.承德医学院临床医学系;2.承德医学院药理学教研室;3.承德市中心医院放化疗中心，河北 承德 067000
  • 收稿日期:2016-09-08 修回日期:2016-11-24 出版日期:2017-04-06 发布日期:2017-04-06
  • 通讯作者: 左彦珍 E-mail:sundayong@163.com
  • 基金资助:

    河北省卫生厅重点课题;河北省高等学校科学技术研究项目;承德市科学技术研究项目

Establishment and identification of radioresistant non-small cell lung cancer model

QI Shu-ya1 SHI Han-fei1 SHI Meng-jie1 ZHANG Meng-hui1 ZUO Yan-zhen 2* SUN Da-yong3   

  1. 1. Department of Clinical Medicine, Chengde Medical University; 2. Department of Pharmacology, Chengde Medical University; 3.Department of Tumor Radiation and Chemotherapy Center, Chengde Central Hospital.  Hebei Chengde 067000, China

  • Received:2016-09-08 Revised:2016-11-24 Online:2017-04-06 Published:2017-04-06
  • Contact: ZUO Yan-zhen E-mail:sundayong@163.com

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摘要：

目的 体外建立非小细胞肺癌A549放疗抵抗模型，为进一步研究放疗抵抗的机制和治疗提供实验基础。 方法 摸索接种细胞数量及诱导照射剂量；采用间歇照射诱导建立放疗抵抗细胞模型RA549；倒置显微镜观察细胞形态；克隆形成实验鉴定RA549的放疗抵抗性。 结果 照射后RA549细胞形态较亲本A549细胞变长、变大，但传代5代后又可恢复；RA549细胞的存活分数明显大于亲本细胞。 结论 间歇照射诱导可以成功建立稳定的、具有放疗抵抗性的肺癌细胞模型。

关键词: 非小细胞肺癌, 放疗抵抗, 克隆形成, 组织化学

Abstract:

Objective To establish a radioresistant model of non-small-cell cancer A549, and to provide the experimental basis for further researchonradioresistance.Methods The cell number and the dose of radiation therapy were confirmed. The intermittent radiation were used to induce the radioresistant cell model. The morphology of cells were observed with an inverted phase contrast microscope. Colony formation was used to identify the radioresistance of the RA549 cell.Results RA549 cells were longer and bigger than A549 cells; surviving fraction of RA549 was significantly increased than A549. Conclusion Intermittent radiation can successfully induce the radioresistant cell model of lung cancer cells.

Key words: Non-small-cell cancer, Radioresistance, Colony formation, Histochemistry