解剖学报

›› 2011, Vol. 42 ›› Issue (2): 236-240.doi: 10.3969/j.issn.0529-1356.2011.02.019

• 论著 • 上一篇    下一篇

Wnt2b/β-catenin/C-myc信号途径在小鼠胚胎心脏中的时空表达

孙莉1* ;丁玉龙1;陈筠1;侯巧燕2   

  1. 1.桂林医学院基础医学院，桂林541004; 2.桂林医学院附属医院病理科，桂林541001
  • 收稿日期:2010-06-09 修回日期:2010-07-19 出版日期:2011-04-06
  • 通讯作者: 孙莉

Temporal and spatial expression of Wnt2b/β-catenin/C-myc signaling pathway in the mouse embryonic heart

  1. 1. College of Basic Medicine of Guilin Medical University, Guilin541004, China; 2. Department of Pathology, Affiliated Hospital of Guilin Medical University, Guilin541001, China
  • Received:2010-06-09 Revised:2010-07-19 Online:2011-04-06
  • Contact: SUN Li

关键词: Wnt2b/β-catenin/C-myc, 胚胎, 心脏, 免疫组织化学, 反转录-聚合酶链式反应, 印迹印迹法, 小鼠

Abstract: Objective To investigate the temporal and spatial expression of Wnt2b/β-catenin/C-myc signaling pathway in mouse embryonic heart. Methods The mouse embryonic hearts taken from gravidity 10 days(G10d), G12.5d, G15d, G17d, G18.5d, and postnatal day(P0d) were used. Immunohistochemistry, RT\|PCR and Western blotting were performed to detect the expressions of Wnt2b, β-catenin and C-myc during different periods of mouse embryonic heart development. Results With the progress of the embryonic heart development, the expressions of Wnt2b, β-catenin and C-myc at the mRNA and protein levels were decreasing gradually, and the mRNA expression of Wnt2b,β-catenin and C-myc showed that they were interrelated statistically (EM>P/EM><0.05).Conclusion These results suggest that Wnt2b/β-catenin/C-myc signaling pathway play important roles in regulating the development of the embryonic heart. And it can predict that C-my

Key words: Wnt2b/β-catenin/C-myc, Embryo, Heart, Immunohistochemistry, RT-PCR, Western blotting, Mouse

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