关键词: 门静脉高压症, 肝动脉, 显微结构, 组织化学, 猪

Abstract: Objective To establish a reliable swine portal hypertension model and explore the morphological properties of hepatic arteries. Methods Liver cirrhosis and portal hypertension were induced by carbon tetrachlorideand fenobarbital (EM>n/EM>=8), 4 pigs served as the control, the hepatic arteries were collected and serially sectioned. The tissue structure, elastic fiber, collagen fiber and smooth muscle were stained by HE, Weigert, Aniline blue and Orange G respectively. The morphological data of hepatic arteries were obtained by the computer image analysis system. Results The portal vein pressure of experimental group was(4.17±1.03) kPa, that of the control group was（1.51±0.79）kPa. The intima and media thickness of hepatic artery was obviously larger than that of the control (EM>P/EM><0.05). The wall thickness and the diameter of hepatic artery were obviously larger than that of the control (EM>P/EM><0.01), the relative percentage of collagen fibers(C) was significantly larger than that of the control (EM>P/EM><0.01), the relative percentage of elastic fibers(E) decreased significantly (EM>P/EM><0.05), the ratio of C and E increased significantly.Conclusion The hepatic artery has suffered extensive morphological remodeling while portal hypertension, and in return affected the h

Key words: Portal hypertension, Hepatic artery, Microstructure component, Histochemistry, Pig