解剖学报

›› 2011, Vol. 42 ›› Issue (4): 446-450.doi: 10.3969/j.issn.0529-1356.2011.04.003

• 神经生物学 • 上一篇    下一篇

p53在蛛网膜下腔出血小鼠脑皮质的表达及其与PI3K/Akt信号通路的关系 

李晓晖1;李冉1; 田艳霞1;崔颖2;崔建忠3;王凯杰3;高俊玲1,4*   

  1. 1.河北联合大学基础医学部，河北 唐山 063000; 2.河北医科大学临床医学系, 石家庄 050017; 3.唐山市工人医院神经外科，河北 唐山 063000; 4.河北省煤矿卫生与安全实验室，河北 唐山 063000
  • 收稿日期:2011-01-20 修回日期:2011-03-11 出版日期:2011-08-06
  • 通讯作者: 高俊玲

Expression of p53 and its relationship with PI3K/Akt signal pathway in subarachnoid hemorrhage in mice cortex

  1. 1.Department of Basic Medicine, Hebei United University, Hebei Tangshan 063000, China;2.Department of Clinical Medicine, Hebei Medical University, Shijiazhuang 050017, China;3.Department of Neurosurgery, Tangshan Gongren Hospital, Hebei Tangshan 063000, China;4.Hebei Province Coal Mine Health and Safety Laboratory, Hebei Tangshan 063000, China
  • Received:2011-01-20 Revised:2011-03-11 Online:2011-08-06
  • Contact: GAO Jun-ling

关键词: 蛛网膜下腔出血, p53, Akt, 免疫组织化学, 免疫印迹法, 小鼠

Abstract: Objective To explore the expression of p53 protein and its relationship with the PI3K/Akt signal pathway in subarachnoid hemorrhage (SAH). Methods SAH was induced in mice by puncture of internal carotid artety. Seventy-two male mice were randomly divided into sham group, SAH group, LY (SAH+LY294002) group. The animals were sacrified at 0, the 3rd, the 6th, the 9th, the 12th and the 24th hour, to observe the expression of p53 and p-Akt in the brain with immunohistochemisty and Western blotting. The morphological changes of the neurons were studied by light microscope. RT-PCR was preformed for detecting the expression change of p53 and p-Akt mRNA. Results Neurons in the hippocampal CA1 region after SAH showed typical apoptotic features under light microscope. The expression of p53 and p-Akt protein was higher in SAH group than in sham group. Compared with SAH group, the expression of p-Akt decreased while the p53 was increased with the extension of time in LY group. Conclusion p53 and p-Akt are increased in cerebral cortex of mice after SAH. PI3K/Akt signal pathway could protect the brain neurons by modulating the p53 expression, which could induce the cell apoptosis.

Key words: Subarachnoid hemorrhage, p53, Akt, Immunohistochemistry, Western blotting, Mouse 

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