解剖学报

›› 2011, Vol. 42 ›› Issue (4): 532-536.doi: 10.3969/j.issn.0529-1356.2011.04.021

• 组织学胚胎学与发育生物学 • 上一篇    下一篇

何首乌饮对运动性疲劳大鼠睾丸组织17β-羟基类固醇脱氢酶表达的影响

郭银1 ;牛嗣云2 ;高福禄3*;曲银娥4;赵秀军4;陈雪4 ;郭凯华1 ;孙一翀1   

  1. 1.承德医学院基础医学部，河北　承德 067000; 2.河北大学医学部组织学胚胎学教研室，河北　保定 071000;3.河北师范大学,石家庄 050016; 4.河北医科大学组织学胚胎学教研室, 石家庄 050017
  • 收稿日期:2011-01-25 修回日期:2011-03-09 出版日期:2011-08-06
  • 通讯作者: 高福禄

Effect of Heshouwuyin on the expression of 17β-hydroxysteroid dehydrogenase in testis tissue of exercise-induced fatigue rats

  1. 1.Department of Basic Science, Chengde Medical College, Hebei Chengde 067000, China; 2.Section of Histology and Embryology, Medical Department of Hebei University，Hebei Baoding 071000，China;3.Hebei Normal University, Shijiazhuang 050016, China; 4.Department of Histology and Embrology, Hebei Medical University, Shijiazhuang 050017， China)
  • Received:2011-01-25 Revised:2011-03-09 Online:2011-08-06
  • Contact: GAO Fu-lu

关键词: 何首乌饮, 运动性疲劳, 睾酮, 17β-羟基类固醇脱氢酶, 免疫组织化学, 免疫印迹法, 反转录-聚合酶链反应, 大鼠

Abstract: Objective To explore the effect of Heshouwuyin on the expression of 17β-hydroxysteroid dehydrogenase (17β-HSD) in testis tissue of exercise-induced fatigue rats. Methods Fifty SD rats were divided randomly into 5 groups, each group had 10 rats including normal control group(A group), Heshouwuyin administered normal group(B group), model control group(C group), Heshouwuyin treated group (D group), Heshouwuyin prevented group (E group). The rats in the C, D and E groups were made exerciseinduced fatigue animal model, and animals were administrated heshouwuyin［20g/(kgI>&#/I>8226;d),contained crude drug 9.6kg/L］, with the exception of E group, animals in E group were administrated heshouwuyin before modeling. The D group was treated with Heshouwuyin by intragastric administration for 60 days after success of modeling. The blood serum concentration of testosterone was detected by radioimmunity, and immunohistochemistry method, Western blotting and RT-PCR were used to observe the differential expression of 17β-HSD. Results 17β-HSD protein expressed in Leydig cells. Compared with C group, the expressions of 17β-HSD protein and mRNA in D, E groups increased (EM>P/EM>0.05)

Key words: Heshouwuyin, Exercise-induced fatigue, Testosterone, 17β-Hydroxysteroid dehydrogenase, Immunohistochemistry, Western blotting, RT-PCR, Rat

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