解剖学报

›› 2011, Vol. 42 ›› Issue (5): 578-581.doi: 10.3969/j.issn.0529-1356.2011.05.001

• 神经生物学 •    下一篇

脑缺血再灌注大鼠半暗带皮质谷氨酸受体相互作用蛋白表达的增加

李薇1;袁华2*;牟翔2;段丽3;曹荣3;瞿丽莉2   

  1. 1.中国人民解放军306医院神经内科，北京100101; 2.中国人民解放军第四军医大学西京医院康复与理疗科，西安710032;3.中国人民解放军第四军医大学基础部神经科学研究所，西安710032
  • 收稿日期:2011-02-21 修回日期:2011-06-01 出版日期:2011-10-06
  • 通讯作者: 袁华

Increase of glutamate receptor interacting protein in the penumbra cortex of rats after transient ischemia and reperfusion

  1. 1.Department of Neurology, the 306th Hospital of PLA, Beijing100101, China;2. Department of Physiotherapy and Rehabilitation, Xijing Hospital, the Fourth Military Medical University, Xi’an710032, China;3. Institute of Neuroscience, the Fourth Military Medical University, Xi’an710032, China
  • Received:2011-02-21 Revised:2011-06-01 Online:2011-10-06
  • Contact: YUAN Hua

关键词: 脑缺血再灌注, 谷氨酸受体相互作用蛋白, 谷氨酸受体2, 免疫组织化学, 免疫荧光, 大鼠

Abstract: Objective To explore the expression of glutamate receptor interacting protein (GRIP) after ischemia-reperfusion in the rat brain. Methods Seventy-two male Sprague Dawley rats were randomly divided into 6 ischemia-reperfusion groups and sham groups with 6 rats per group. Middle cerebral artery occlusion (MCAO) was achieved with the use of an intraluminal filament to occlude the left middle cerebral artery, and reperfused 2 hours later. At the 1st, 3rd, 6th, 12th, 24th and 72th hour after reperfusion or sham operation, the rats from each group were decapitated. Immunohistochemistry and double immunofluorescence were used to observe the distribution of GRIP in the cortex and also its relationship with glutamate receptor 2 (GluR2). Results Compared with the sham-operated group which showed few positive neurons, GRIP positive neurons increased obviously from 3 hours after ischemia-reperfusion in the ischemic penumbra cortex, mainly on the dendrites at the early stage and moved to the cell body lately. Double immunohistochemical staining showed that the GRIP positive cells were co-stained with GluR2 neurons. Conclusion GRIP increased and accelerated to transfer GluR2 to the membrane after ischemia-reperfusion which may protect the neurons in the penumbra zone from the AMPA receptor mediated excitotoxic injury.

Key words: Brain ischemia-reperfusion, Glutamate receptor interacting protein, Glutamate receptor 2, Immunohistochemistry, Immunofluorescence, Rat

中图分类号: