解剖学报

›› 2011, Vol. 42 ›› Issue (5): 635-639.doi: 10.3969/j.issn.0529-1356.2011.05.012

• 细胞和分子生物学 • 上一篇    下一篇

17β-雌二醇对高同型半胱氨酸诱导人成骨肉瘤MG63细胞损伤的保护作用及机制

陈学秋1,2;戴纪男1;刘承杏1;边立功1;郭家智1;孙俊1;桂莉3*;陆地1*   

  1. 1.昆明医学院人体解剖学教研室, 昆明650500; 2.云南省中医学院人体解剖学教研室, 昆明650500;3.云南省第三人民医院内分泌科，昆明650011
  • 收稿日期:2011-01-08 修回日期:2011-03-04 出版日期:2011-10-06
  • 通讯作者: 桂莉;陆地

Protection effects and mechanism of 17β-estradiol in high homocysteine induced injury of human osteosarcoma MG63 cells EM>in vitro/EM>

  1. 1.Department of Anatomy, Kunming Medical University, Kunming650500, China; 2.Department of Anatomy, Yunnan University of Traditional Chinese Medicine, Kunming650500,China; 3.Department of Endocrinology, the 3rd People’s Hospital of Yunnan Province, Kunming650011,China
  • Received:2011-01-08 Revised:2011-03-04 Online:2011-10-06
  • Contact: GUI Li; LU Di

关键词: MG63细胞, 17β-雌二醇, 高同型半胱氨酸, 活性氧簇, 荧光探针, Hoechst 33342/ PI核双染法, 免疫印迹法

Abstract: Objective To explore the protection effects and molecular mechanisms of 17β-estradiol (17β-ESUB>2/SUB>) in high homocysteine (HHcy) induced injury of human osteosarcoma MG63 cells EM>in vitro/EM>. Methods Human osteosarcoma MG63 cells cultured EM>in vitro/EM> were treated with HHcy in different concentrations at different time after 17β-estradiol intervention treatment. The levels of reactive oxygen species (ROS ) were detected with a 2′,7′-dichloro-fluorescin diacetate probe (HSUB>2 /SUB>DCFDA ). The apoptotic and necrotic cells were determined by double nuclear staining with Hoechst 33342/PI. The expression levels of Caspase-3 and Bax were measured with Western blotting. Results 1.17β-ESUB>2/SUB>significantly suppressed the HHcy induced production of ROS in MG63 cells and the extent of suppression was positively associated with the dose and time. 2.17β-ESUB>2/SUB> significantly prevented MG63 cells from apoptosis or necrosis induced by HHcy and down-regulated t

Key words: MG63 cell, 17β-estradiol, High homocysteine, Reactive oxygen species, Fluorescence probe, Hoechst/PI nuclear staining, Western blotting

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