解剖学报

›› 2011, Vol. 42 ›› Issue (6): 787-791.doi: 10.3969/j.issn.0529-1356.2011.06.014

• 肿瘤生物学 • 上一篇    下一篇

肿瘤疫苗Ad-hDCT抑制黑色素瘤B16细胞在C57BL/6小鼠脑内增殖

李健1; 蔡华丹1;杨美娟1;WAN Yong-hong 2;郝钰1*   

  1. 1.北京中医药大学基础医学院人体形态学系, 北京 100029;2.Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada L8N3Z5
  • 收稿日期:2011-04-11 修回日期:2011-05-12 出版日期:2011-12-06
  • 通讯作者: 郝钰

Ad-hDCT (tumor vaccine) inhibiting the proliferation of intracranial B16 melanoma cells in C57BL/6 mice

  1. 1.Department of Human Morphology, Preclinical College, Beijing University of Chinese Medicine, Beijing 100029, China; 2.Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N3Z5, Canada
  • Received:2011-04-11 Revised:2011-05-12 Online:2011-12-06
  • Contact: HAO Yu

关键词: Ad-hDCT, 黑色素瘤细胞系B16, 脑肿瘤, 免疫组织化学, 流式细胞术, C57BL/6小鼠

Abstract: Objective To observe inhibitive effect on the intracranial B16 melanoma cells in C57BL/6 mice by vaccination with Ad-hDCT. Methods Forty C57BL/6 mice were divided to 2 groups: Ad-BHG control group and Ad-hDCT treatment group. B16-F10 cells were planted into the brain by mouse stereotaxie apparatus. General observation, flow cytometry, paraffin sections with HE staining，frozen sections with CD8 and F4/80 immunohistochemistry detection were adopted in this study.Results Seven days after vaccination with Ad-hDCT, the level of hDCT specific CD8+INF-γ+ T cells increased significantly in the peripheral blood of the mice (P 0.01 vs Ad-BHG group). Compared to 100% endpoint of Ad-BHG blank vector control, the survival rate was 60% in Ad-hDCT immunized group after B16 cells plantation in parenchyma 16 days. The mice behaviour was normal. Histological analysis indicated that no tumor cells proliferated in the vaccinated mice. CD8+ T cells were detected in the tumor mass to recognize and kill tumor cells in vaccinated animals. Furthermore, much more microglia were found around the tumor area in the brain of the vaccinated mice.Conclusion Ad-hDCT can elicit a strong cytotoxic T lymphocyte (CTL) response in C57BL/6 mice, leading to protection against

Key words: Ad-hDCT, B16 melanoma cell, Brain tumor, Immunohistochemistry, Flow cytometry, C57BL/6 mouse

中图分类号: