›› 2012, Vol. 43 ›› Issue (2): 262-267.doi: 10.3969/j.issn.0529-1356.2012.02.022

• 组织学胚胎学和发育生物学 • 上一篇    下一篇

大鼠实验性胃溃疡自愈期间垂体三叶因子3的变化

张小俊1; 吕洋1; 张文静1; 张静1; 邵素霞2; 吴靖芳1*    

  1. 1.河北北方学院组织学胚胎学教研室,河北 张家口075000; 2.河北医科大学组织学胚胎学教研室,石家庄050017
  • 收稿日期:2011-09-17 修回日期:2011-11-20 出版日期:2012-04-06
  • 通讯作者: 吴靖芳

Changes of trefoil factor 3 in hypophysis during healing of experimental gastric ulcer in rats

  1. 1.Department of Histology and Embryology, Hebei North University, Hebei Zhangjiakou 075000, China; 2.Department of Histology and Embryology, Hebei Medical University, Shijiazhuang 050017, China
  • Received:2011-09-17 Revised:2011-11-20 Online:2012-04-06
  • Contact: WU Jing-fang

关键词: 三叶因子3, 垂体, 实验性胃溃疡, 反转录-聚合酶链式反应, 免疫组织化学, 大鼠

Abstract: Objective To explore the function of trefoil factor 3(TFF3) during the self-healing of experimental gastric ulcer in rats,and to observe the changes of TFF3 in hypophysis and blood serum. Methods The expression and changes of TFF3 peptide and mRNA in hypophysis at 42 gastric ulcer,21 saline and 6 normal rats were detected by immunohistochemical SP and RT-PCR methods respectively.The content of TFF3 peptide of blood serum in different phase was detected by ELISA.In situ hibridazation method (ISH) was used to detect the location of TFF3 mRNA in hypophysis. Results TFF3 immunoreactive profiles were mainly located in some cells of adenohypophysis(AP),and neurohypophysis(NP),but TFF3 mRNA only in some cells of AP by ISH.The average absorbance(AA) values increased from the day 1 to 6 after gastric ulcer,reached its penk on the 6th day,then a little decreased on the day 10, increased on day 14 again,then decreased on the day 23,but remained at a high level compared with the control group(EM>P /EM>0.01 orEM> P/EM> 0.05).In NP,there was a high level undulation from day 2 to 23 with the highest on the 14th day(EM>P/EM> 0.01 or EM>P /EM>0.05).TFF3mRNA transcription was detected in every group.The AAof TFF3/GAPDH was obviously higher in gastric ulcer group on the day 2to 23 compared with the control group(EM>P/EM> 0.01

Key words: Trefoil factor 3, Hypophysis, Experimental gastric ulcer, RT-PCR, Immunohistochemistry, Rat

中图分类号: