Protective effect of hydrogen sulfide on brain damage in rats with subarachnoid hemorrhage

doi:10.16098/j.issn.0529-1356.2015.03.002

Abstract

Abstract:

Objective To explore the effect of hydrogen sulfide (H₂S) to the brain injury in rats with subarachnoid hemorrhage (SAH). Method Thirty experimental rats were randomly divided into Control group (n=10), SAH model group (n=10) and NaHS (100 mg/Kg)group (n=10),The experimental rats of the H₂S group were injected NaHS 100 mg /Kg body weight in intraperitoneal and after surviving for 24 hours to do the neurological assessment and take the whole brain. The expression changes of GFAP, S-100b, Bcl-2, C-fos, caspase -3, MMP-9 were detected with fluorescent immunocytochemistry and Western blot method. Results Neurological assessment showed that severe neurological symptoms was significantly relieved in hydrogen sulfide group. GFAP and S-100b have a high expression in SAH rats group and were significantly decreased in H₂S pretreatment group. The expression of Bcl-2 and caspase -3 in SAH group were high than in the control group but the expression of Bcl-2 was increased in H₂S pretreatment group than SAH group, and caspase -3 was decreased in H₂S pretreatment group. Western blot analysis showed that the expression of c-fos and MMP-9 were increased in SAH group and was significantly decreased in H₂S pretreatment group. Conclusion The hydrogen sulfide have an significant protective effect on brain injury after SAH and its mechanism may be improved the function of glial cells and apoptotic pathways.

LIU Yan-mei ZHU Qian LINGHU Yan WANG Jian-qiang LIU Jun LI Wen-jie WANG Min-chen WU Kai-yun*. Protective effect of hydrogen sulfide on brain damage in rats with subarachnoid hemorrhage[J]. AAS, 2015, (3): 297-303.

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