Comparison of methods for preparing mouse ileum vascular transparent specimen

doi:10.16098/j.issn.0529-1356.2015.06.021

Abstract

Abstract:

Objective To compare the advantages and disadvantages of the method for preparing mouse ileum transparent vascular specimens with different fillers and transparent manner. Methods The viscosity coefficients(VCs) of the filler through a simple capillary tube were measured. Eight mice were divided into 4 groups. The blood vessel was washed by the modified perfusion method, injected with different fillers, and treated with different transparency. The refractive indexes(RIs) of ileum tissues and transparent agent were compared. Results Both of the VCs and filling effect of material nitrocellulose were better than other groups. At 20℃, the difference value of RIs between ileum tissues and transparent solutions were 2.2×10^-3 nD (Glycerin) and 65.8×10^-3 nD (Transparent-fixative), respectively.Conclusion The specimen which was filled with nitrocellulose and treated with transparentfixative method has the better effect.

Key words: Ileum, Vascular transparent, Refractive index, Mouse

XU Jia-yi HE Zi-wei SU He-jing* ZHANG Jie Lü Meng-xiang WANG Zhao-long TAI Hong-xiang. Comparison of methods for preparing mouse ileum vascular transparent specimen[J]. AAS, 2015, 46(6): 848-851.

References

［1］Dai ZhSh, Li ZhH, Yang ChJ, et al. Techniques for Human Specimen Preparation ［M］. Shanghai: Fudan University Press, 2008：188-200. (in Chinese)
戴正寿，李忠华，杨成杰，等. 解剖学标本制作技术［M］. 上海：复旦大学出版社，2008：188-200.
［2］Tai HX, Wang ZhL, Gao WZh, et al. Improvement of the method of preparing animal skeleton transparent specimens ［J］. Journal of Tianjin Medical University, 2014, 20(6):494-495. (in Chinese)
台红祥，王兆龙，高卫真，等. 动物骨骼透明标本制作方法的改进［J］. 天津医科大学学报，2014，20（6）：494-495.
［3］Zheng N, Gong J, Yu SB, et al. A combination method for preparing casting and transparent liver specimen ［J］. Clin Anat, 2010, 23(5):559-562.
［4］Huang HL, Liu Zh, Ji RJ, et al. Preparation of hand artery transparent specimen ［J］. Chinese Journal of Anatomy, 2014,37(2):265. (in Chinese)
黄会龙，刘镇，姬瑞娟，等. 手动脉透明标本的制作［J］. 解剖学杂志，2014，37（2）：265.
［5］Chen GSh, Lu HS, Zhang WH, et al. Preparation and clinical application of rabbit systemic artery cast specimen ［J］. Progress of Anatomical Sciences, 2015,37(1):110-111. (in Chinese)
陈耿树，卢华森，张伟华，等. 家兔全身动脉铸型标本的制作及临床应用［J］. 解剖科学进展，2015，37(1):110-111.
［6］Du XH, Liu X, Liu Y, et al. Observation on the architecture of subpleural pulmonary microvasculature in Bactrian camel (Camelus Bactrianus) under sem ［J］. Acta Anatomica Sinica, 2006,37(1): 87-90. (in Chinese)
杜晓华，刘霞，刘英，等. 双峰驼胸膜下肺微血管构筑的扫描电镜观察［J］. 解剖学报，2006，37(1):87-90. 
［7］Xi HJ, Zhang PF, Luo JS. The concept of the cavernous sinus and its anatomical observation ［J］. Acta Anatomica Sinica, 2005,36(4): 395-399. (in Chinese)
席焕久，张鹏飞，罗俊生. 海绵窦的解剖学概念及相关结构的观察［J］. 解剖学报，2005，36(4):395-399. (in Chinese)
［8］Lou JSh, Xu ShM, Liu X. Functional Experiment ［M］. Beijing: People’s Medical Publishing House, 2007:174-176. (in Chinese)
娄建石，徐淑梅，刘欣. 机能学实验［M］. 北京：人民卫生出版社，2007:174-176.
［9］Zheng EL, Zou JH, Huang JZh. Study on application of fetal vascular cast filling material ［J］. Anatomy Research, 2011,33(4):320-321. (in Chinese)
郑二来，邹锦慧，黄剑真. 胎儿血管铸型填充材料的应用研究［J］. 解剖学研究，2011，33(4):320-321.
［10］Feng ChL, Jia F. Improvement of measurement method for fluid viscosity coefficient ［J］. Experimental Technology and Management, 2005,22(4):32-33, 51. (in Chinese)
冯朝岭，贾芳. 流体黏滞系数测量方法的改进［J］. 实验技术与管理，2005，(22)4：32-33, 51.
［11］Tian ChSh, Ma HT. Improvement of measurement method for fluid viscosity coefficient ［J］. Journal of Qinghai University (Natural Science), 2006,24(1):76-78. (in Chinese)
田春山，马洪涛. 液体黏滞系数测量方法的改进［J］. 青海大学学报(自然科学版)，2006，24(1):76-78.
［12］Zheng H, Li YF, Xu GQ. The selection and processing of transparent specimen material ［J］. Journal of Qiqihar Medical School, 2001, 22(1):114-115. (in Chinese)
郑辉，李云峰，徐贵清. 透明标本材料的选择和处理［J］. 齐齐哈尔医学院学报，2001，22(1): 114-115.

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