Effects of annexin A5 low expression on migration and invasion of HeLa cells

doi:10.16098/j.issn.0529-1356.2016.04.009

Abstract

Abstract:

Objective To investigate the effects of annexin A5 (ANXA5) low expression on HeLa cell migration and invasion. Methods HeLa cells were divided into four groups, which were interference group, negative control group, and transfection reagent control group, and blank control group. RT-PCR and Western blotting were used to identify the inhibition efficiency at 72 hours after transfection. Cell scratch method and Transwell experiment were used to detect the ability of migration and invasion of HeLa cells in each group. E-cadherin and matrix metalloproteinase(MMP)-9 mRNA and protein were determined by RT-PCR and Western blotting respectively. Results HeLa cell ANXA5 expression on both mRNA and protein level was suppressed significantly(P<0.05). Compared with the negative control group, the abilities of migration and invasion of HeLa cells in the interference group were increased remarkably (P<0.05). The E-cadherin mRNA and protein expression of HeLa cells in interference group decreased while MMP-9 increased when compared with negative control group (P<0.05). Conclusion The siRNA which targets ANXA5 can highly suppress ANXA5 expression. ANXA5 knockdown promotes migration and invasion of HeLa cells via upregulation of E-cadherin and downregulation of MMP-9.

Key words: Annexin A5, HeLa cell, Migration, Invasion, Cell wound scratch assay, Transwell assay

WANG Xiao-jie YANG Yan-jie2TIAN Huan-na LIAGN Xiu-jun CHEN Long LI Xin. Effects of annexin A5 low expression on migration and invasion of HeLa cells[J]. Acta Anatomica Sinica, 2016, 47(4): 487-492.

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