Hypoxia promotes lung adenocarcinoma A549 cells migration by upregulating acetyl-CoA carboxylase 1

doi:10.16098/j.issn.0529-1356.2021.02.015

Abstract

Abstract:

Objective To investigate the mechanism of hypoxia to promote human lung adenocarcinoma A549 cells migration through acetyl-CoA carboxylase 1 (ACC1). Methods Lung adenocarcinoma A549 cells were treated with hypoxia (5% O₂). Transwell migration assay was used to detect cell migration ability. Western blotting was used to detect ACC1 expression and epithelial-mesenchymal transition (EMT) related protein expression. Results Compared with the normoxia (control group), hypoxia treatment promoted the migration of A549 cells (P<0.01), ACC1 expression was up-regulated after hypoxia treatment (P<0.01), and vimentin expression was detected to increase significantly (P<0.05), E-cadherin expression decreased (P<0.01); Compared with the control group, migration of A549 cells was inhibited (P<0.05), vimentin expression was down-regulated (P<0.05), and E-cadherin expression increased after knocking down ACC1(P<0.01). After ACC1 was knocked down, the differences between the numbers of migration of A549 cells under normoxia and 5% O2 conditions and the expressions of vimentin and E-cadherin were not statistically significant (P>0.05). After linoleic acid (LA) supplementation, the hypoxia-induced migration promotion of A549 cells was restored. Conclusion Hypoxia can promote the migration and EMT transformation of lung adenocarcinoma A549 cells by up-regulating the expression of ACC1.

Key words: Hypoxia, Lung adenocarcinoma, Migration;Acetyl-CoA carboxylase 1, Western blotting

CLC Number:

R734.2

JIN Jia-hao ZHAO Bao-sheng LIU Dan-hui LIU Y-zhen. Hypoxia promotes lung adenocarcinoma A549 cells migration by upregulating acetyl-CoA carboxylase 1[J]. Acta Anatomica Sinica, 2021, 52(2): 258-263.

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