Effects of ephedrine on histological structure, activities of total antioxidant capacity and catalase in the myocardial tissue of mice

doi:10.16098/j.issn.0529-1356.2016.04.014

Abstract

Abstract:

Objective To study the poisonous effects of ephedrine on the myocardial tissue. Methods Sixty mice were randomly divided into control group and ephedrine group，thirty mice in each group.The mice in ephedrine group were injected with ephedrine at the doses 0.0315 g/kg，0.0477 g/kg，0.0564 g/kg (×0.2ml)，respectively，twice per day for 15 days，and the mice in control group were injected with equal amount of saline.Changes of body weight and heart weight were weighed, the activities of creatine kinase (CK)、creatine kinase-MB (CK-MB) in plasma and the activities of total antioxidant capacity (T-AOC), catalase (CAT) as well as the content of malondialdehyde (MDA) in myocardial tissue were detected by colorimetry，the changes of the myocardial tissue were observed by optical microscopy. Results Both the body weight and heart weight in ephedrine group were lower than that in the control group.Myocardial tissue of mice appeared different degree of damage after injected ephedrine，myocardial cell arrangement confusion，intercalated disc gap widened，and myocardial fiber breakage were observed.The activities of plasma CK and CK-MB in ephedrine group were significantly higher than that in control group (P＜0.01).The activities of T-AOC and CAT in ephedrine group were significantly lower compared to the control group (P＜0.05 orP＜0.01). MDA contents in ephedrine group were significantly higher than that in control group (P＜0.01). Conclusion Ephedrine affects the shape and structure of myocardial tissue in mice，which may be correlated with activity decreased of T-AOC and CAT and content increased of MDA.

Key words: Ephedrine, Myocardium, Histological structure, Total antioxidant capacity, Catalase, Creatine kinase, Colorimetry, Mouse

LIU Ting-ting PENG Jing LI Chong-yang YU Shi-yuan*. Effects of ephedrine on histological structure, activities of total antioxidant capacity and catalase in the myocardial tissue of mice[J]. AAS, 2016, 47(4): 516-520.

References

［1］Lü XH.The present situation of the application of ephedrine drugs［J］.Shanxi Medical Journal, 2002, 31(5): 363-365.(in Chinese)
吕向华. 麻黄素相关药物的应用现状［J］.山西医药杂志，2002，31（5）：363-365.
［2］Li JH.Ephedrine and its clinical application［J］.Herald of Medicine，2001，20（1）：11-12.(in Chinese)
李继珩.麻黄素及其临床应用现状［J］.医药导报，2001，20（1）：11-12.
［3］Sun J, Li ChY，Yu ShY，et al.Effects of ephedrine on structure and polypeptides in the liver of filial mice［J］.Acta Anatomica Sinica，2012，43(3)：398-404. (in Chinese)
孙隽，李重阳，俞诗源，等.麻黄素对仔鼠肝脏组织结构和相关活性物质的影响［J］.解剖学报，2012，43(3)：398-404.
［4］Wang JJ，Li ChY，Yu ShY，et al. Effects of ephedrine on histological structure, activities of superoxide dismutase， catalase，and content of maleic dialdehyde in kidney of filial mice［J］. Chinese Journal of Zoology，2011，46(6)：118-125.(in Chinese)
王锦锦，李重阳，俞诗源，等.麻黄素对仔鼠肾组织结构和SOD、CAT活力及MDA含量的影响［J］.动物学杂志，2011，46(6)：118-125.
［5］Wang Y，Li ChY，Yu ShY，et al. Effects on the structure of cerebral temporal lobe cortex and capability of learning and memory of filial mice after administration of heroin and ephedrine［J］.Acta Anatomica Sinica，2009，40（5）：724-731.(in Chinese)
王昱，李重阳，俞诗源，等.注射海洛因、麻黄素对仔鼠大脑颞叶皮层结构及学习记忆能力的影响［J］.解剖学报，2009，40（5）：724-731.
［6］Hou P，Yang L. Effects of ephedrine，β-asarone and higenamine on calcium concentration and cell membrane calcium channels in rat ventricular myocytes［J］. Journal of China Medical University, 2013, 42(3):201-203.(in Chinese)
侯平，杨丽.麻黄碱、β-细辛醚和去甲乌药碱对大鼠心肌细胞钙离子浓度和细胞膜钙通道的影响［J］.中国医科大学学报，2013，42（3）：201-203.
［7］Shekelle PG，Hardy ML，Morton SC，et al.Efficacy and safety of ephedra and ephedrine for weight loss and athletic performance: a meta-analysis［J］. JAMA，2003，289(12)：1537-1545.
［8］Caldwell RW，Goldberg LI.An evaluation of the vasodilation produced by mephentermine and certain other sympathomimetic amines［J］.J Pharmacol Exp Ther，1970，172(2)：389-396.
［9］Grandy JL，Hodgson DS，Dunlop CI，et al. Cardiopulmonary effects of ephedrine in halothane-anesthetized horses［J］. J Vet Pharmacol Ther，1989，12(4)：389-396.
［10］Lin Q, Ma HX.The protection for myocardial injury mice by CVB3 of K-CoxB-JN compound extract［J］.Chinese Journal of Gerontology, 2015,35（12）:3199-3201.(in Chinese)
林清，马会霞.K-CoxB-JN复方提取物对CVB3感染小鼠心肌损伤的保护作用［J］.中国老年学杂志，2015,35（12）:3199-3201.
［11］Bi FJ, Zhang H.Protective effect of catalpol on isoproterenol-induced myocardial injury in rats［J］. Journal of China Medical University,2013，42（3）：244-248.(in Chinese)
毕方杰，张虎.梓醇对异丙肾上腺素诱导的大鼠心肌损伤的保护作用及机制研究［J］.中国医科大学学报，2013，42（3）：244-248.
［12］Liu ZhY. Advance of research of sports training and creatine kinase［J］.Journal of Tianjin Institute of Physical Education,1999,14（1）：30-32. (in Chinese)
刘振玉.运动训练与肌酸激酶研究进展［J］.天津体育学院学报，1999,14（1）：30-32.
［13］Chen Y，Yao YZh.Recognition of ephedra［J］. Journal of Liaoning University of Traditional Chinese Medicine, 2008，10(12)：48-49.(in Chinese)
陈艳，姚映芷.麻黄之再认识［J］.辽宁中医药大学学报，2008，10(12)：48-49.
［14］Kanaya N，Satoh H, Seki S, et al.Propofol anesthesia enhances the pressorrez sponse to intravenous enedrine［J］.Anesth Analg，2002，94(5)：1207-1211.
［15］Li Y，Kong LQ，Gao H, et al.Organic free radical and disease research progress［J］.Progress in Veterinary Medicine，2008，29(4)：85-88. (in Chinese)
李勇，孔令青, 高洪，等.自由基与疾病研究进展［J］.动物医学进展，2008，29(4):85-88.
［16］Yuan ChF. To contrast the effects of selenium tea with nonselenium tea on total antioxidative capacity in aging mice induced with D-galactose［J］.Journal of Hubei Institute for Nationalities(Medical Edition)，2004，21(3)：51-53. (in Chinese)
袁成福.硒茶和非硒茶对D-半乳糖致衰老小鼠总抗氧化能力的对照研究［J］.湖北民族学院学报（医学版），2004，21(3)：51-53.
［17］Wang YCh，Yu ShY，Wang Y，et al. Effects of X-ray on structure of cerebral occipital lobe cortex and activities of SOD,CAT and content of MDA in cerebrum of filial mice［J］.Acta Anatomica Sinica，2010，41(5)：641-648. (in Chinese)
王元春，俞诗源，王昱，等.X射线辐射对仔鼠大脑枕叶皮层结构及大脑超氧化物岐化酶、过氧化氢酶活性及丙二醛含量的影响［J］.解剖学报，2010，41(5)：641-648.
［18］Liu XY，Li ChY，Yu ShY，et al. Effects of ephedrine on ovarian histological structure［J］. Acta Anatomica Sinica，2014，45（3）：398-404. (in Chinese)
刘小媛，李重阳，俞诗源，等.麻黄素对卵巢组织结构的影响［J］.解剖学报，2014，45（3）：398-404.
［19］Li L，Yu ShY，Ma JB，et al.Effects of melamine on histological structure and activities of SOD，CAT and content of MDA in kidney of filial mice［J］.Acta Anatomica Sinica，2011，42(1)：110-117.(in Chinese)
李丽，俞诗源，马建博，等.三聚氰胺对仔鼠肾脏组织结构和超氧化物岐化酶、过氧化氢酶活性及丙二醛含量的影响［J］.解剖学报，2011，42(1)：110-117.

[1]	HONG Xiao-min LI San-qiang CUI Qin-yi ZHENG Run-yue YANG Meng-li LUO Ren-li LI Qian-hui. Nuclear factor-κB signaling pathway and gender differences in alcoholic liver fibrosis [J]. Acta Anatomica Sinica, 2024, 55(1): 55-61.
[2]	YUAN Lei YANG Zhi-wei YANG Hui LIU Zheng-hai HE Jie WAN Wei. Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice [J]. Acta Anatomica Sinica, 2024, 55(1): 25-31.
[3]	MA Ting-ting CHEN Jian-hong LIU Ai-cui LI Hai-ning. Role of inhibiting lncRNA TUG1 to down-regulate nucleotide binding oligomerization domain like receptor protein 1 inflammasome in delaying the progression of Alzheimer’s disease [J]. Acta Anatomica Sinica, 2024, 55(1): 32-42.
[4]	ZOU Cheng-gong FENG Hao CHEN Bing TANG Hui SHAO Chuan SUN Mou YANG Rong HE Jia-quan . Research on the dynamic changes of neurological dysfunction and cognitive function impairment in traumatic brain injury [J]. Acta Anatomica Sinica, 2024, 55(1): 43-48.
[5]	ZHANG Qin YANG Jun-xia JIANG Qing-song. Effect of fosinopril on angiotensin converting enzyme 2 and vascular endothelial growth factor in diabetic retinopathy mice [J]. Acta Anatomica Sinica, 2023, 54(6): 628-634.
[6]	CHEN Zi-xuan SI Li-na SHU Wei-han ZHANG Xin WEI Chen-yang CHENG Lu-yang YANG Song-he QIAO Yue-bing. Mechanism of melatonin regulation of PI3K/Akt/mTOR signaling in hypothalamus delaying the initiation of puberty in female mice mice [J]. Acta Anatomica Sinica, 2023, 54(6): 644-651.
[7]	LIU Zhe-chuan LI Kun MA Shuai-nan MENG Jia-qi WANG Yan-qin. Effects of liraglutide on inflammation and mitochondrial fusion/division in Parkinson’s disease model of mice induced by paraquat [J]. Acta Anatomica Sinica, 2023, 54(6): 676-681.
[8]	LIU Ye CHU Ya-nan XU Ce-lu HE Jia-cheng SU Bing-yin TAI Hao-ran. Roscovitine rescuing neuronal loss and neuroinflammation in brain regions associated with Parkinson’s disease mice [J]. Acta Anatomica Sinica, 2023, 54(6): 635-643.
[9]	WEI Chun-chun WANG Ping LIN Fang-xing MA Xian-hua ZHANG Wei-ping XIE Zhi-fang. An improved fixation method for preparing mouse brown adipose tissue for transmission electron microscopy [J]. Acta Anatomica Sinica, 2023, 54(6): 738-743.
[10]	YAN Kun WU Xiao-lin LIU Ying-juan GE Ke-li1 REN Lei-ming LI Hong-yun . Effect of cerebrotein hydrolysate-Ⅰon intestinal microflora regulation of mice with Parkinson’s disease [J]. Acta Anatomica Sinica, 2023, 54(5): 497-504.
[11]	Ling GU ZHANG Ping SONG KeXin Na Li Hong-yu Chen Zhang Jie CONG Jing ZHAI XiaoYue GU Ling ZHANG Ping SONG Ke-xin LI Na CHEN Hong-yu ZHANG Jie CONG -Jing ZHAI Xiao-yue . Morphometry of the ureteric bud branching in the developing mouse kidney [J]. Acta Anatomica Sinica, 2023, 54(5): 593-598.
[12]	HE Ke-ke LI Yuan-di WEN Ting-hao ZHU Jie GAO Jie HU Rong HAN Feng SU Min . Erythrocyte membrane-associated protein inhibiting Th17 cell differentiation via IL-6/STAT3/ROR-γt signaling pathway in experimental autoimmune encephalomyelitis mice encephalomyelitis mice [J]. Acta Anatomica Sinica, 2023, 54(5): 538-545.
[13]	FAN Wen-juan CHEN Xu-dong CHEN Yong-fang YANG Xu-guang JIN Shao-ju ZHAO Zhi-jun DENG Jin-bo. Developmental comparison between cerebral organoids in vitro and body’s cortices in vivo [J]. Acta Anatomica Sinica, 2023, 54(4): 383-391.
[14]	ZHANG Yi-zhou LI Sha MI Shi-xiong ZUO Hong-chun ZHANG Qian-qian LI Han-lin LEI Zi-han ZHANG Dong-ze CUI Hui-xian. Effect of estradiol regulating sortilin-related receptor A expression on hippocampal spine density and synaptic protein expression via estrogen receptor of mouse [J]. Acta Anatomica Sinica, 2023, 54(3): 261-268.
[15]	LIU Ke-ting CHEN Bin-bin SHUI Yu-lian SHI Qing-ming XIAO Li. Neuroprotective effect of exogenous hydrogen sulfide and the expression of matrix metalloproteinases-9 and Caspase-1 in mice model of Parkinson’s disease [J]. Acta Anatomica Sinica, 2023, 54(3): 289-295.