Effects of ephedrine on histological structure, activities of total antioxidant capacity and catalase in the myocardial tissue of mice

doi:10.16098/j.issn.0529-1356.2016.04.014

Abstract

Abstract:

Objective To study the poisonous effects of ephedrine on the myocardial tissue. Methods Sixty mice were randomly divided into control group and ephedrine group，thirty mice in each group.The mice in ephedrine group were injected with ephedrine at the doses 0.0315 g/kg，0.0477 g/kg，0.0564 g/kg (×0.2ml)，respectively，twice per day for 15 days，and the mice in control group were injected with equal amount of saline.Changes of body weight and heart weight were weighed, the activities of creatine kinase (CK)、creatine kinase-MB (CK-MB) in plasma and the activities of total antioxidant capacity (T-AOC), catalase (CAT) as well as the content of malondialdehyde (MDA) in myocardial tissue were detected by colorimetry，the changes of the myocardial tissue were observed by optical microscopy. Results Both the body weight and heart weight in ephedrine group were lower than that in the control group.Myocardial tissue of mice appeared different degree of damage after injected ephedrine，myocardial cell arrangement confusion，intercalated disc gap widened，and myocardial fiber breakage were observed.The activities of plasma CK and CK-MB in ephedrine group were significantly higher than that in control group (P＜0.01).The activities of T-AOC and CAT in ephedrine group were significantly lower compared to the control group (P＜0.05 or P＜0.01). MDA contents in ephedrine group were significantly higher than that in control group (P＜0.01). Conclusion Ephedrine affects the shape and structure of myocardial tissue in mice，which may be correlated with activity decreased of T-AOC and CAT and content increased of MDA.

Key words: Ephedrine, Myocardium, Histological structure, Total antioxidant capacity, Catalase, Creatine kinase, Colorimetry, Mouse

LIU Ting-ting PENG Jing LI Chong-yang YU Shi-yuan. Effects of ephedrine on histological structure, activities of total antioxidant capacity and catalase in the myocardial tissue of mice[J]. Acta Anatomica Sinica, 2016, 47(4): 516-520.

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