Effect of chemokine 2 on the formation of lipid on liver regeneration

doi:10.16098/j.issn.0529-1356.2016.05.022

Abstract

Abstract:

Objective To elucidate the effect of chemokine 2 (CCL2) on liver regeneration and its mechanism. Methods Rats were randomly divided into 3 groups with 10 rats in each group. The plasmids were transferred into rats by the hydraulic gene transfer technology. Transfection efficiency was observed under a fluorescence microscope after 6 hours. The weight of regenerated liver was weighed, and the liver regenerating rate, together with the liver index were calculated to observe the liver regeneration. Liver function was observed by the content of alanine aminotransferase, aspartate aminotransferase and total bilirubin in serum. Sultan Ⅳ staining was used to observe the lipid accumulation, and Real-time PCR to detect the expression of genes related to lipid metabolism; The expression of phosphorylated mitogen extracellular kinase 1/2 (p-MEK1/2) and phosphorylated extra-cellular-regulated kinase l/2 (p-ERKl/2) were detected by Western blotting. Results After 6 hours, the positive rate of green fluorescent protein in each group was more than 30%. After transfection with pEGFP-N1-CCL2, liver regenerating rate, liver index, alanine amiotransferase(ALT), aspartate aminotransferase(AST) and total bilirubin(TBIL) were all higher than those of the pEGFP-N1 group. As the transgenic time was prolonged, the expression of the adipose related genes increased, more lipid drops appeared, and the expression of p-MEK1/2 and p-ERKl/2 increased. Conclusion Chemokine 2 may promote liver regeneration by increasing lipid synthesis via the MEK/ERK pathway.

Key words: Chemokine 2, Lipid synthesis, Liver regeneration, Sudan Ⅳ, Real-time PCR, Western blotting, Rat

XING Xue-kun WU Hong-yan2LIN Jun-tang FENG Hui-gen YUAN Zhi-qing. Effect of chemokine 2 on the formation of lipid on liver regeneration[J]. Acta Anatomica Sinica, 2016, 47(5): 697-702.

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