Expression and clinical significance of Ezrin in the serous ovarian cancer

doi:10.16098/j.issn.0529-1356.2017.01.010

Abstract

Abstract:

Objective To investigate the expression feaure of Ezrin in the normal ovarian epithelial tissues and the serous ovarian cancer tissues and evaluate the relationships between Ezrin expression and the clinicopathological characteristics of ovarian cancer patient. Methods The relative Ezrin mRNA- expression was detected by Real-time PCR in 40 specimens of ovarian carcinoma and 27 specimens of normal ovarian epithelial fresh frozen tissues. A total of 134 specimens of paraffin-embeded ovrian cancer tissues and 27 specimens of normal ovarian epithelial tissues were collected,and the Ezrin protein level was examined by immunohistochemistry. The correlation of Ezrin with the clinicopathological characteristics of patients with ovarian cancer was analyzed by SPSS 20.0. Results Both the RNA and protein levels of Ezrin were significantly reduced in ovarian cancer compared to the normal control(P<0.05). Statistical analysis revealed that Ezrin expression had no correlation with age, cytoreduction satisfactory degree and sensitivity of chemotherapy. However there was a significant difference in ezrin protein levels with the disease-free and overall survival (P<0.05). Although ezrin did not serve as an independent predictor for disease progression (HR 1.406, P>0.05) and overall survival (HR 1.260, P>0.05), the expression of Ezrin was negatively correlated with clinical stage,differentiation, and Oentum majus metastasis (P<0.05). Conclusion The down-regulation of Ezrin may play a substantial role in the pathophysiology of serous ovarian cancer. Therefore, it may be used as the potential pre-diagnostic and prognostic marker of ovarian cancer and direct the clinical treatment in the future.

Key words: Ezrin, Serous ovarian cancer, Oentum majus metastasis, Progression free survival, Overal survival, Real-time PCR, Human

XIE Zhe LI Lan-fang LUO Qing-ya LIU Yi LI Gui-qin WANG Geng-chao MAO Cheng-yi YI Ping. Expression and clinical significance of Ezrin in the serous ovarian cancer[J]. Acta Anatomica Sinica, 2017, 48(1): 54-60.

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