Distal-less homeobox 2/ forkhead box O3a distribution and expression in the development of rat cerebral cortex

doi:10.16098/j.issn.0529-1356.2017.06.004

Abstract

Abstract:

Objective To investigate the expression of distal-less homeobox 2(Dlx2) and forkhead box O3a(FoxO3a) in developing cortex of SD rats. Methods Real-time PCR and immunohistochemical staining were performed to study the changes in Dlx2/FoxO3a expression. Results Dlx2/FoxO3a mRNA were expressed in embryonic day 11(E11)-postnatal day 1(P1), while the expression peak of FoxO3a mRNA was significantly earlier than Dlx2 mRNA. The expression of Dlx2 mRNA was significantly increased at E15-E17, and this high level expression was maintained until P1. The expression of Dlx2/FoxO3a mRNA showed a consistent trend in E15-P1. At E19,Dlx2 gene showed high expression of mRNA but no protein expression. Conclusion Dlx2 and Fox03a genes are expressed in the cerebral cortex of the late embryos,and their expression trend is consistent.The distributions of the two genes are changed with the cortical stratification.

Key words:

Distal-less homeobox 2, Forkhead box O3a, Cerebral cortex, Embryo development, Real-time PCR, Immunohistochemistry, Rat

MU Ya-ting LI Yan GUO Qiong WANG Lei. Distal-less homeobox 2/ forkhead box O3a distribution and expression in the development of rat cerebral cortex[J]. Acta Anatomica Sinica, 2017, 48(6): 658-662.

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