Effect of heroin on histological structures and its antioxidant enzyme in mice retinae

doi:10.16098/j.issn.0529-1356.2017.06.005

Abstract

Abstract:

Objective To investigate the effect of heroin on mouse retina. Methods Sixty mice were randomly divided into 2 groups：the control group and the heroin group. The heroin group was intraperitoneally injected with heroin at the increasing doses (2，3，4 g/L) continuously(1-5，6-10 and 11-15 d) for 15 days (twice a day，×0.2 ml)，and the control group was injected with equivalence saline. The changes of the body and eyeball were weighted. The structural changes of the retinal tissue of mice were observed by microscopy. Activities of superoxide dismutase(SOD) and the content of malonaldehyde(MDA) in retinal tissue were detected by colorimetry. Immunohistochemistry was used to measure the expressions of Bax and tumor necrosis factor α(TNF-α) protein in retina. Results Both body weight and eyeball weight of the heroin group were lower than that of the control group. The retinal tissues in mice appeared different degrees of damage after heroin injection. The ganglion cells were decreased and appeared cavity degeneration，the inner nuclear layer and the outer nuclear layer arrangement confusion and loose. The activity of SOD was significantly reduced while the content of MDA was significantly increased in the retinal tissue. The expressions of Bax and TNF-α protein in retina were significantly increased. Conclusion Heroin may reduce antioxidant capacity and increase the lipid peroxidation of retinal tissue，inducing the apoptosis of retinal cells and inflammation，and having a certain damage effect for retina.

Key words:

Heroin, Retina, Histological structure, Antioxidant, Immunohistochemistry, Mouse

GOU Tao-ran LI Chong-yang OU Rui ZHU Jia-ning YU Shi-yuan. Effect of heroin on histological structures and its antioxidant enzyme in mice retinae[J]. Acta Anatomica Sinica, 2017, 48(6): 663-668.

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