Effect of N-cadherin overexpression on protrusion formation and catenins expression in mouse neuroblastoma N2a cells

doi:10.16098/j.issn.0529-1356.2018.05.006

Abstract

Abstract:

Objective To analyse the effects of N-cadherin overexpression on cell morphology and catenins expression by using mouse neuroblastoma N2a cells. Methods The mouse N-cadherin gene was cloned to pCAG-MCS-EGFP plasmid and transfected into N2a cells by Lipofectamine 3000. The empty vector pCAG-MCS-EGFP and the overexpression vector pCAG-N-Cad-EGFP transfected group were used as control group and experimental group, respectively. Cell scratche and cell adhesion experiments were used to detect the effects of N-cadherin overexpression on the migration and adhesion of N2a cells. Immunofluorescence was used to detect the effect of N-cadherin overexpression on the morphology of N2a cells. The expression of catenin family was detected by Western blotting. Results Compared with the control group, overexpressing N-cadherin promoted the protrusion formation of N2a cells, which led to the increase and elongation of protrusion in N2a cells. N-cadherin overexpression enhancea cell adhesion and inhibited migration. N-cadherin overexpression resulted in the expression level of α-catenin, β-catenin and p120 protein significantly increased (P<0.05), while γ-catenin expression decreased but the difference was not significant (P>0.05). In addition, the cell aggregation phenomenon was observed when N-cadherin overexpression. Conclusion N-cadherin overexpression promotes protrusion formation and adhesion ability in N2a cells, but inhibits cell migration, regulates the expression of the family of catenin and promotes cell aggregation.

Key words: N-cadherin, Mouse neuroblastoma N2a cell, Cell protrusion, Catenin, Cell adhesion, Immunofluorescence, Mouse

ZHU Shao-yi GUAN Li-hong YANG Ci-qing ZHANG Bi-chao LI Qiu-ling LIN Jun-tang. Effect of N-cadherin overexpression on protrusion formation and catenins expression in mouse neuroblastoma N2a cells[J]. Acta Anatomica Sinica, 2018, 49(5): 598-604.

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