Up-regulation of serine protease inhibitor Kazal type 1 expression in human lung adenocarcinoma and promotion of proliferation of cancer cells

doi:10.16098/j.issn.0529-1356.2019.02.009

Abstract

Abstract:

Objective To explore the relationship between serine protease inhibitor Kazal type 1(SPINK1) expression and survival time of lung adenocarcinoma (LAC) and its effect on the growth of LAC cells. Methods The SPINK1 expressions in 285 cases of lung adenocarcinoma tissues and adjacent tissues were detected by Real-time PCR and immunohistochemical staining. The relationship between SPINK1 expression and clinicopathological features and prognosis of lung adenocarcinoma was analyzed. Human LAC cells were treated with lentivirus vector with SPINK1 or SPINK1 siRNA. Green fluorescent protein(GFP) expression was observed under microscope. SPINK1 expression was detected by enzyme-linked immunosorbent assay (ELISA) or Real-time PCR. The growth of LAC cells was evaluated by cell count and colony formation assay. Results The levels of SPINK1 mRNA and protein level in lung adenocarcinoma were up-regulated (P<0.0001). The overall survival time in the group of SPINK1 high expression was shorter than that of SPINK1 low expression (50.0 months vs 64.5 months, P<0.001). In SPINK1 overexpression group, SPINK1 protein was up-regulated in the cultures of LAC cells (P<0.05), and the ability of proliferation and colony formation was up-regulated (P<0.05). In SPINK1 knockdown group, SPINK1 mRNA was down-regulated in LAC cells (P<0.05), and the ability of proliferation was decreased (P<0.05). Conclusion High expression of SPINK1 in lung adenocarcinoma suggests poor prognosis. SPINK1 promotes the proliferation of LAC cells.

Key words: Lung adenocarcinoma, Serine protease inhibitor Kazal type 1, Cell proliferation, Real-time PCR, Human

LU Chang-chang HUANG Yan-yan XU Li-yun HE Jian-ying QIU lei LE Han-bo. Up-regulation of serine protease inhibitor Kazal type 1 expression in human lung adenocarcinoma and promotion of proliferation of cancer cells [J]. Acta Anatomica Sinica, 2019, 50(2): 192-200.

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