Objective The traditional round incision or cross incision brain harvesting method  can not meet the requirements of protecting the donor’s remains. In this study, the method  of brain removal through a posterior incision on the scalp of both ears was proposed, which effectively protected the donor’s remains.   Methods Adopting the incision 2.0 cm above the external occipital protuberance to the most front edge of the auricle to obtain a complete brain.   Results The incision did not involve the head and face skin, which was small and conducive to suture repair and reduce exudation.   Conclusion The incision effectively protects the donor’s remains, and it will be conducive to the establishment and development of the brain bank.