Objective  To investigate the effects of inhibition of chemokine C-X-C motif receptor 7(CXCR7) expression on the proliferation, migration, differentiation and mitochondrial function of human urine-derived stem cells(USCs) under hypoxia.  Methods  CXCR7 expression was inhibited by siCXCR7 and detected by Real-time PCR and Western blotting in hypoxia group treated with 3%O2 for 48 hours. Cell proliferation was detected by clonal formation assay and cell growth curve. Cell migration ability was detected by scratch assay and Transwell assay. Alkaline phosphatase, alizarin red, oil red O and alcian blue staining were used to detect the multidirectional differentiation ability of cells. Mitochondrial function was evaluated by JC-1 fluorescent probe, adenosine triphosphate(ATP) and reactive oxygen species(ROS). Results  Compared with the normal oxygen group, the expression of CXCR7 in USCs in hypoxia group was significantly up-regulated, and hypoxia promoted the proliferation, migration and clonogenesis of USCs. SiCXCR7 inhibited the expression of CXCR7 and inhibited the effects of hypoxia on the proliferation, migration and clonogenesis of USCs, but had no effect on cell differentiation. Hypoxia treatment increased mitochondrial membrane potential and ATP levels, and decreased the production of reactive oxygen species, while CXCR7 inhibition decreased mitochondrial membrane potential and ATP production. Conclusion  Hypoxia may enhance mitochondrial function of USCs through the CXCR7 signaling pathway, thereby promoting cell proliferation and migration.