Abstract: Objective We compare the effects of fibrinogen gel and chitin on BMSCs to chondrocytes differentiation in order to explore the relationship between threedimensional scaffold and cartilage tissue engineering seed cells BMSCs differentiation. Methods BMSCs together with chitin and fibrin gel complexes were cultured EM>in vitro/EM> and implanted into rat’s articular cartilage defect location. After 14 days EM>in vitro/EM> culture, HE staining, toluidine blue staining and type II collagen immunohistochemical staining were performed; After transplantation EM>in vivo/EM>, for 2 weeks, 4 weeks and 6 weeks, morphology observations, expression of cartilagespecific protein analysis and BMSCs EM>in vivo/EM> tracer method were performed. We analyzed the differentiation of BMSCs into chondrocytes by statistical methods. Results The comparison of positive cell rate of type II collagen immunohistochemical staining in BMSCs-fibrin gel group and BMSCs-chitin group which were cultured EM>in vitro/EM>, showed no significant difference with control group. Integrated absorbance(IEM>A/EM>) change rate of toluidine blue staining and type II collagen immunohistochemical staining in BMSCsfibrin gel group which was cultured EM>in vivo/EM>, showed significantly different with other groups and control group.Conclusion The results showed that EM>in vitro/EM> fibrin gel or chitin has very weak induction of BMSCs to cartilage

Key words: Bone-marrow-derived mesenchymal stem cells, Chondrocyte, Fibrinogen gel, Chitin, Immunohistochemistry, Rat