Acta Anatomica Sinica

AAS ›› 2013, Vol. 44 ›› Issue (3 ): 334-338.doi: 10.3969/j.issn.0529-1356.2013.03.008

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Effect of mouse Sertoli cells on human umbilical cord mesenchymal stem cells proliferation

ZHANG Fen-xi  HONG Yan*  LIANG Wen-mei   

  • Received:2012-07-09 Revised:2013-01-11 Online:2013-06-06 Published:2013-07-16

Abstract:

Objective To observe the regulatory effect of Sertoli cells (SCs) on proliferation in human umbilical cord mesenchymal
stem cells (hUCMSCs) in vitro. Methods hUCMSCs and SCs were isolated, cultured and identified in vitro. A co-culture system was
established by culturing SCs in the Transwell insert and hUCMSCs on the plastic plates(the co-culture group). hUCMSCs culture in
DMEM-F12 medium was used as the control group. hUCMSCs culture in DMEM-F12 medium supplemented IL-3 and GM-CSF served as the
cytokine group. Proliferation, cell cycle analyse and surface marker molecules of hUCMSCs were studied by flow cytometry and
immunocytochemistry. Ultrastructures of the cultured cells were observed by transmission electron microscopy. Results Compared
with the control, proliferation of hUCMSCs in the co-culture- and cytokine-group increased significantly in the co-culture group
(P<0.05). The expression of CD29 and CD105 on the surface of hUCMSCs in the co-culture and the cytokine group also increased.
Cell cycle analysis showed that there were a few cells arrested at quiescent phases in the co-culture and cytokine groups,
especially in the cytokine group. In the coculture group and the control group，hUCMSCs may displayed the ultrastructural features
of the primitive cells by transmission electron microscopy. Conclusion Proliferation of hUCMSCs may be promoted as co-culture with
SCs and still kept their stem cell properties after the co-culture.

Key words: Umbilical cord mesenchymal stem cell, Sertoli cell, Co-culture, Culture in vitro, Immunohistochemistry, Flow cytometry, Mouse

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