Acta Anatomica Sinica

AAS ›› 2013, Vol. 44 ›› Issue (3 ): 349-352.doi: 10.3969/j.issn.0529-1356.2013.03.011

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Expression of germ cell nuclear factor and Oct-4 before and after in vitro differentiation of mouse P19 Cell line

WANG Ju 1, 2  XIONG Cheng-liang2  GAO Qi-yun1  LI Hong-gang2  WANG Miao1  ZHAO Kai2  ZHOU Zong-yao 1*   

  1. 1. Department of Histology and Embryology, School of Medicine, Shihezi University, Xinjiang Shihezi 832002, China;
    2. The Family Planning Institute, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China
  • Received:2012-06-18 Revised:2013-01-28 Online:2013-06-06 Published:2013-07-16

Abstract:

Objective To detect the expressions of Oct-4 and germ cell nuclear factors(GCNF) before and after inducing P19
cells to differentiation. Methods The P19 cells were induced to differentiation with ATRA treatment.The immunofluorescence
staining and RT-PCR techniques were used to detect the expression of GCNF and Oct-4 before and after differentiation of mouse
P19. Results The intensities for Oct-4 immunofluorescence positive staining were stronger in non-differentiated P19 cells than
that in the differentiated P19 cells. The intensities for GCNF immunofluorescence positive staining were weaker in non-differentiate
P19 cells than that in the differentiated P19 cells. Conclusion GCNF is involved in the in vitro differentiation progress of
the malignant germ cell tumors. GCNF be inhibited through the Oct-4 gene expression in vitro differentiation of P19 cells.

Key words: Oct-4, Germ cell nuclear factor, P19 cell, Differentiation, Indirect immunofluorescence, RT-PCR