Expression of Nrf2 induced by sodium arsenite in mouse pancreatic β cells

doi:10.3969/j.issn.0529-1356.2013.03.010

Abstract

Abstract:

Objective To test the expressions of Nrf2 and phase II detoxifying enzyme genes induced by sodium arsenite (NaAsO₂)
in mouse pancreatic β-cell MIN6.Methods MIN6 cells were exposed to 4μmol/L NaAsO₂ for 2, 6, 12, 18, 24hours. Nrf2 protein
levels in Nuclear factions, cytosolic factions and whole-cell of MIN6 cells were measured with Western blotting. mRNA expressions
of Nrf2 and phase II detoxifying enzyme genes were measured by Real-time PCR. Results Expression of Nrf2 protein was significantly
increased after 4μmol/L NaAsO₂ exposure did reached the peak at 2hours, then decreased gradually. Nrf2 protein expressions in
nuclear factions were consistent with Nrf2 levels of the whole MIN6 cell in the time-dependent fasion. But 4μmol/L NaAsO₂ exposure
did not induce a robust increase in Nrf2 mRNA. The induction of some phase II detoxification enzyme genes, including NAD(P)H:quinone
oxidoreductase 1 (Nqo1), heme oxygenase 1(Homx-1), were time-dependently increased in MIN6 cells. Nqo1 and Homx-1 mRNA expressions
were obviously increased after 2hours NaAsO₂ exposure and went higher at 6hours, then decreased gradually. Conclusions Upon exposure
to NaAsO2, Nrf2 is stabilized and translocated into the nucleus, and then activates transcription of various detoxification
enzyme genes in mouse pancreatic β-cells.

Key words: Arsenic, Sodium arsenite, Diabetes, Pancreatic β-cell, Nrf2, Phase II detoxification enzyme, Western blotting, Mouse

YANG Bei* CHEN Xue DING Jin-lan. Expression of Nrf2 induced by sodium arsenite in mouse pancreatic β cells[J]. AAS, 2013, 44(3 ): 345-348.

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