Effect of Chinese traditional medicine on activities of antioxidant enzyme and special protein of kidney in ephedrine addiction mice

doi:10.3969/j.issn.0529-1356.2013.03.017

Abstract

Abstract:

Objective To research the effect of Chinese traditional medicine on ephedrine addiction mice. Methods A model of
addiction in ephedrine dependent mice was established by intraperitonealy injected escalation doses ( 2.0, 3.0, 4.0 g/L ) of
ephedrine 15 days，and then for withdrawal by irrigate to stomach with escalation doses(20.0，30.0，40.0g/L) astragalus and
ligustici chuanxiong Hort decoction and Chinese medicine compound recipe．The samples on withdrawal at 5，10 and 15 days. The
content of bloodurea nitrogen(BUN) and malonaldehyde(MDA)，and activities of superoxide dismutase(SOD) and catalase(CAT) were
detected by spectrophotometer. The expression of Bax protein and TGF-β1 were examined by immunohistochemical methods. Structures
of and the kidney were observed by optical microscopy after irrigation of Chinese medicine. Results SOD and CAT activities the
of experimental control group were lower than natural control group. While MDA content increased, the expression intensity of
Bax protein and TGF-β1 of the experimental control group were higher than the natural control group. The content of blood serum
BUN increased structures of the kidney appeared severity harm. The SOD and CAT activity of kidney in astragalus ligusticum
chuanxiong Hort and Chinese medicine compound group increased，while the content of MDA decrease．The degeneration of kidney
tissue structure significantly decreased in astragalus ligusticum chuanxiong Hort and Chinese traditional medicine compound
groups. The intensity of positive expression of Bax protein and TGF-β1 intensity decreased，The content of blood serum BUN
markedly reduced. Conclusion Astragalus，ligusticum chuanxiong Hort and Chinese medicine compound recipe may improve the ability
of cell anti-oxidation, promote the cell repairing and reduce cell damage.

Key words: Chinese medicine compound, Ephedrine, Enzyme activity, Bax protein, Kidney, Spectrophotometer, Immunohistochemistry, Mouse

LI Chong-yang WANG Jin-jin YU Shi-yuan* SUN Jun . Effect of Chinese traditional medicine on activities of antioxidant enzyme and special protein of kidney in ephedrine addiction mice[J]. AAS, 2013, 44(3 ): 372-378.

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