AAS ›› 2013, Vol. 44 ›› Issue (6 ): 861-864.doi: 10.3969/j.issn.0529-1356.2013.06.022

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Isolation and culture of first trimester human trophoblast cell

ZUO Yan-zhen2 ZHANG Kong-yan3 LI Yu-hong 1* XU Qian4 MAO Xiao-dan4 TAN Yu-si4   

  1. 1. Department of Pathology, Chengde Medical College; 2. Department of Pharmacology; 3. Department of Geriatrics, Affiliated Hospital of Chengde Medical College; 4. Institute of Basic Medical, Chengde Medical College, Hebei Chengde 067000, China)
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  • Received:2013-01-14 Revised:2013-05-10 Online:2013-12-06 Published:2013-12-06
  • Contact: LI Yu-hong E-mail:120950413@qq.com

Abstract:

Objective To research a simple method for purification of first trimester human trophoblast cell in vitro.Methods Whole first trimester human chorionic tissues were isolated with trypsin/collagenase. HE staining and immunocytochemistry methods were used to detect the tyophoblast cells purity. Results Cytokeratin-7 positive cells were more than 90%. Conclusion First trimester human Trophoblast cells can be obtained through the simple method and may offer enough cells for later experiments.

Key words: Tyophoblast cell, First trimester, Primary cell culture, Percoll gradient, Human