Effects of TWEAK antisense oligonucleotide on the proliferation and invasive capability of the human colon cancer cell line SW480

doi:10.3969/j.issn.0529-1356.2014.01.014

Abstract

Abstract:

Objective To explore the effects of TNF-like weak inducer of apoptosis(TWEAK) antisense oligonucleotide on the proliferation and invasive capability of the human colon cancer cell line SW480. Methods Colon cancer SW480 cells were transfected with synthesized TWEAK antisense oligodeoxynucleotide (ASODN) and scrambled oligodeoxynucleotide (SCODN). The cells were divided into control group, LF control group, ASODN group and SCODN group; MTT was applied to detect the inhibition of tumor cell proliferation, flow cytometry to examine the cell cycle distribution, ELISA to investigate the expression of soluble TWEAK and its receptor Fn14 in supernatant of cell culture fluid, Western blotting and immunofluorescence cytochemistry(IF) to detect the expression of soluble TWEAK and its receptor Fn14 and Transwell invasion assay to observe the changes on invasive capability of colon cancer cells. Results There was no significant difference on proliferation between the control group, LF control group and SCODN group (P>0.05). Compared with the control group, the proliferation rate of ASODN group tumor cells was inhibited obviously (P<0.05) in a dosage-time dependent manner. The ratio of G2+M phase cells was remarkably higher than that of control group after transfection with TWEAK ASODN (P<0.05). The expression rates of TWEAK and its receptor Fn14 in both cell culture fluid and cells were lower than those of control group, and the difference was of statistical significance (all P<0.05). The inhibition rate of infiltration was 31.39% after transfection with TWEAK ASODN, higher than those of control groups (P<0.05). The expression of TWEAK and its receptor Fn14 in cell culture fluid and cells was closely correlated (all P<0.05). Conclusion TWEAK ASODN may affect the occurrence and development of tumor probably by inhibition of binding between TWEAK and its receptor Fn14, and inhibition of TWEAK protein expression may restrain the proliferation and infiltration of the human colon cancer cell line SW480.

Key words: Tumor necrosis factor-like weak inducer of apoptosis, Colon cancer, Proliferation, Infiltration, MTT assay, Flow cytometry, Human

ZHANG Yan-xin* ZHAO Hong-jun SONG Wen-gang GAO Feng-lan Lü Hui-fang. Effects of TWEAK antisense oligonucleotide on the proliferation and invasive capability of the human colon cancer cell line SW480[J]. AAS, 2014, 45(1): 74-79.

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