Effects and mechanisms of periostin overexpression on invasion and migration of the nasopharyngeal carcinoma 6-10B cell line

doi:10.3969/j.issn.0529-1356.2014.04.011

Abstract

Abstract:

Objective To explore the effects and mechanisms of periostin overexpression on migration and invasion of nasopharyngeal carcinoma(NPC) cell line. Methods The recombinant plasmids ［pCMV-neo (+)-periostin］ and control plasmids ［pCMV-neo (+)］ were transfected into 6-10B cells using lipofectamine 2000TM reagent. The expression of periostin was detected with PCR and Western blotting. Transwell chamber invasion assay was employed to assay the migration and invasion of 6-10B cells before and after transfection. A gelatin zymogram was used to detect the activity of MMP-2 and MMP-9 in cultivated supernatant of 6-10B cells before and after transfection. The expression of integrin-α_vβ₅ was detected by immunohistochemistry(IHC) in 6-10^Bperiostin cells, 6-10^Bvectorcells and 6-10B cells as well as normal nasopharyngeal mucosa (NNM) and NPC and at the same time periostin also was detected by immumohistochemistry in NNM and NPC, and densitometry analysis using image-pro plus 6.0 software, and the correlation between periostin and integrin-α_vβ₅ on NPC was assayed with statistics. Results Over expression of periostin promoted cell migration and invasion. The expression levels of integrin-α_vβ₅ in primary NPC and 6-10Bperiostin cells were significantly higher than those in NNM and 6-10^Bvector, 6-10B cells. The expression in NPC of integrin-α_vβ₅showed positively correlated with the expression of periostin (r=0.682, P<0.01). Conclusion Periostin plays an important role in regulation of cell migration and invasion probably by combining with integrin-α_vβ₅to improve the activities of MMPs.

Key words: Periostin, Nasopharyngeal cancer, Metastasis, transfection, Transwell, Immunohistochemistry, Human

WANG Hui-jie SHI Jin-feng XIE Yuan-jie ZENG Gu-qing DU Ya-lan HUANG Xing-qiong LONG Zhi-feng YU Jiang-dong LI Mei-xiang*. Effects and mechanisms of periostin overexpression on invasion and migration of the nasopharyngeal carcinoma 6-10B cell line[J]. AAS, 2014, 45(4): 500-506.

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