Effects of p38-2G4 high-expression on the proliferation and erythriod differentiateon of murine erythroleukemia cells

doi:10.3969/j.issn.0529-1356.2014.05.015

Abstract

Abstract:

Objective To explore the effect of mouse proliferation-associated protein 2G4 (p38-2G4) high-expression on the proliferation and erythriod differentiation of murine erythroleukemia (MEL) cells. Methods To establish the recombinant lentivirus vector p38-2G4-pLJM1, the p38-2G4-pLJM1 was cotransfected into HEK293T cells to obtain lentivirus with pCMV-VSV-G and pCMV-dR8.2. Lentivirus were infected into MEL cells to establish the stably p38-2G4 high-expressed MEL cells. Western blotting was used to analyse the high-expression efficiency. MTT assay and benzidine staining were applied to detect the cell viability and hemoglobin synthesis of the stable cell line in presence/absence of inducers. Results Western blotting showed that the p38-2G4 high-expression stable cell strain had a higher expression of p38-2G4 as compared to the control group (MEL)（P<0.05）. MTT result showed that there was no difference between the p38-2G4 high-expression cell strain and the control group（P>0.05）, but the hemoglobin synthesis had been reduced as compared to the control group（P<0.05）. Conclusion p38-2G4 high-expression does not affect the cell viability of MEL cells, but inhibits the erythriod differentiation of MEL cells in three independent experiments.

Key words: p38-2G4, Murine erythroleukemia, Lentiviral transfection, High-expression, Western blotling, Mouse

ZHU Xiao-fang SHI Ming-ming YANG Zu-li ZHAO Fu-kun ZHANG Shi-fu*. Effects of p38-2G4 high-expression on the proliferation and erythriod differentiateon of murine erythroleukemia cells[J]. AAS, 2014, 45(5): 670-674.

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