Expression of embryonic stem cell-associated factor Nanog in gliomas and its significance

doi:10.3969/j.issn.0529-1356.2013.01.001

Abstract

Abstract:

Objective To explore the expression of embryonic stem cell-associated factor Nanog in human gliomas and its significance. Methods Forty cases of human gliomas were examined. The expression of Nanog was analysed by immunohistochemistry, reverse transcription polymerase chain reaction and Western blotting.Double immunofluorescence staining was used to investigate the coexpression of Nanog and markers of cancer stem cells or embryonic stem cell pluripotency-related gene Oct4. Results We found a positive correlation between the expression levels of Nanog and tumour malignancy. Immunohistochemistry showed that Nanogexpressed in both the nuclei and the cytoplasm of glioma cells. Double immunofluorescence staining revealed that more than 50% of Nanog-positive cells coexpressed the putative CSC markers CD133 and Nestin. More than 95% Nanog positive cells also expressed embryonic stem cell pluripotency-related gene Oct4. Conclusion The result suggests that Nanog is mainly expressed in cancer stem cells in gliomas and positively correlated with the malignancy of gliomas. Nanog may play an important role in the progress of gliomas.

Key words: Glioma, Cancer stem cell, Nanog, Immunofluorescence, Human

CLC Number:

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