Acta Anatomica Sinica

AAS ›› 2013, Vol. 44 ›› Issue (3 ): 339-344.doi: 10.3969/j.issn.0529-1356.2013.03.009

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Comparison of effect on the induction of three methods of inducing CD73+ adipose-derived mesenchymal stem cells into myocardial cells

QI Li-jie1  GUO Kang2  LI Qiong 1,3  GUO Zhi-kun 1*   

  1. 1.Key Open Laboratory for Medical Tissue Regeneration of He’nan Province，Xinxiang Medical University, He’nan Xinxiang 453003, China;
    2. Department of Oncology，the Third Affiliated Hospital，He’nan Xinxiang 453003, China; 3. Department of Histology and Embryology，
    Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430000, China
  • Received:2012-08-17 Revised:2012-11-23 Online:2013-06-06 Published:2013-07-16

Abstract:

Objective To isolate and purify CD73 positive cell subsets from the mixed adipose-derived mesenchymal stem cells
（ADMSCs）, and to compare the differentiation potential of CD73 cell to cardiomyocyte by three methods. Methods ADMSCs of
1-3-month-old mice were isolated and cultured in vitro. ADMSCs subpopulations for CD73+ and CD73- were selected by flow cytometry,
and were cultured separately. Two groups of the sorted cells were induced into the myocardium by using 5-aza, myocardial tissue
lysate and 5-aza+ myocardial tissue lysate respectively. Immunohistochemistry was used to detect the effect of myocardium after
the induction of three ways. Results Unsorted ADMSCs differentiated into adipocytes and ostcoblasts. Sorted CD73+ADMSCs possessed
a perfect differentiating potential for cardiaomyocyte. All three induction methods inducted CD73+ ADMSC into cardiomyocytes.
The differentiation rate of myocardial cells which induced by 5-aza was (22.99±6.72)%, the rate by myocardium lysate was
(14.12±5.42)%, and the rate by 5-aza+myocardium lysate was (26.94±6.11)%. Of the three methods, 5-aza+myocardium lysate was
the best method for inducting CD73+ ADMSCs to myocardial cell(P<0.05). Conclusion CD73+ADMSCs may differentiate to cardiomyocyte
more easily than CD73-ADMSCs. Chemical induction factors with the simulated myocardial microenvironment in vitro may efficiently
induce ADMSCs to differentiate into cardiomyocyte.

Key words: CD73, 5-aza, Myocardial tissue lysate, Adipose-derived mesenchymal stem cell, Cell culture, Immunofluorescence, Mouse