Lhx8 promotes the differentiation of hippocampal neural stem cells into cholinergic neurons in vitro

doi:10.3969/j.issn.0529-1356.2013.06.002

Abstract

Abstract:

Objective To construct eukaryotic expression vector containing full length cDNA of rat Lhx8 gene. Methods Full length cDNA of rat Lhx8 gene was acquired by PCR method and digested by EcoRI and BamHI. The digested gene fragment was inserted into eukaryotic expression vector pEGFP-C3 and identified by DNA sequencing. The recombinant plasmid was transfected into hippocampal neural stem cells (NSCs) by liposome. Results DNA sequencing demonstrated that the insertion fragment in recombinant plasmid was right, and recombinant plasmid pEGFP-C3-Lhx8 was transfected into NSCs. After 7 days, the number of ChAT positive cells in plasmid transefection group was significantly higher than the negative control group. Conclusion Eukaryotic expression vector pEGFP-C3- Lhx8 is constructed successfully, and Lhx8 promotes the differentiation of NSCs into ChAT positive cellsin vitro.

Key words: Lhx8, Neural stem cell, Hippocampus, Gene expression, PCR, Rat

TIAN Mei-ling ZHU Pei-pei JIN Guo-hua* LI Hao-ming QIN Jian-bing SHI Jin-hong. Lhx8 promotes the differentiation of hippocampal neural stem cells into cholinergic neurons in vitro[J]. AAS, 2013, 44(6 ): 735-739.

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