Tissue affinity of bone marrow mesenchymal stem cell and the therapeutic transplantation for neurodegenerative diseases

doi:10.3969/j.issn.0529-1356.2014.06.003

Abstract

Abstract:

Objective To study the affinity of bone marreow mesenchymal stem cells (BMSCs) to various tissues and the possibility for therapeutic transplantation to cure neurodegenerative diseases with BMSCs. Methods The hippocampal slices, liver slices and lung slices were co-cultured with BMSCs to investigate BMSCs affinity to the tissues above, and immunohistochemistry (NeuN and GFAP) was carried out to understand the differentiation of BMSCs into neurons and glial cells under microenvironment of hippocampal slices, liver slices and lung slices. Results BMSCs had a highest affinity with hippocampal slices in contrast with the slices of liver and lung. The BMSCs’ temporal affinity on hippocampal slice was observed as well. At P7, BMSCs had the highest affinity with hippocampus, but there was a time-related decrease afterward. Differentiation of BMSCs occurred on hippocampal slices of both WT and Tg mice. Under the induction of hippocampal microenvironment, BMSCs differentiated into nervous cells and glial cells, but there was no significance between WT and Tg mouse. Conclusion BMSCs may provide an opportunity for the therapeutic transplantation to cure the neurodegenerative diseases and brain injuries. The time-related decrease of cell adhesion to hippocampus shows that the early stem cell transplantation is necessary.

Key words: Bone marrow mesenchymal stem cell, Affinity, Neurodegenerative disease, Immunohistochemistry, Mouse

WEN Ya-nan NIU Yan-li HU Lei FU Xing LI Jin-ju WU Ping* DENG Jin-bo*. Tissue affinity of bone marrow mesenchymal stem cell and the therapeutic transplantation for neurodegenerative diseases[J]. AAS, 2014, 45(6): 742-748.

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