Protection of Angelica sinensis polysaccharide on liver histological structure in mice injured by ephedrine

doi:10.16098/j.issn.0529-1356.2015.03.016

Abstract

Abstract:

Objective To investigate the protection of Angelica sinensis polysaccharide(ASP) on ephedrine induced hepatic injury in mice. Methods Seventy-two mice were randomly divided into 4 groups：the natural control group，experiment control group，ASP1 group， and ASP2 group.The experimental control group and ASP groups were intraperitoneally injected with a 4.0g/L of ephedrine 0.2ml twice a day for 15 days， and the natural control group was injected with a same amount of saline.The ASP groups were irrigated to stomach with 0.3ml Angelica sinensispolysaccharide solution (12.5g/L，25.0g/L)1 hour after ephedrine injection，the natural control group and experiment control group were irrigated to stomach with a same amount of saline.The mice were sacrificed at 5 day，10day，and 15 day after treatment，respectively.The morphological observation was used to observe pathological changes of hepatic tissues.Activities of hepatic histologicalsuperoxide dismutase（SOD）and plasma γ-glutamyltransferase(γ-GT), alkaline phosphatase (AKP), glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminase(AST)，as well as content of malonaldehyde（MDA）were detected by colorimetry.The expressions of Bax protein and Caspase-3 protein were measured by immunohistochemistry in liver of mice. Results There were various degree damages in hepatic tissues，hepatic cords were disorganized，liver cells were loose and liver sinusoid expanded，the activity of γ-GT、AKP、ALT、AST in plasma were significantly increased，the activity of SOD in liver tissue was significantly reduced，the content of MDA in liver tissue was increased significantly，the expression of Bax and Caspase-3 protein in liver were significantly increased.Compared with the experimental control group，pathological damage of liver was significantly improved，the activity of plasma γ-GT, AKP, ALT, AST were significantly reduced，the activity of SOD in liver tissue was increased significantly，however the content of MDA was significantly decreased and the expressions of Bax and Caspase-3 protein in liver were reduced in Angelica sinensis polysaccharide group. ConclusionAngelica sinensis polysaccharide can improve liver tissue antioxidant capacity and promote reparation of cell injury and protect hepatic injury which is induced by ephedrine.

MENG Ru LI Chong-yang YU Shi-yuan* YUAN Jing WEI Ping-ting. Protection of Angelica sinensis polysaccharide on liver histological structure in mice injured by ephedrine[J]. AAS, 2015, (3): 379-386.

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