›› 2016, Vol. 47 ›› Issue (1): 139-144.

• technology and methodology • Previous Articles    

Established the 3D anatomy interaction system of zebrafish (Danio rerio)

  

  • Received:2015-07-02 Revised:2015-10-23 Online:2016-02-06 Published:2016-02-06
  • Contact: Jin-Xing LIN E-mail:linjinxing83@163.com

Abstract: Objective The aim of this paper was to make a software of three-dimension (3D) anatomical interaction system in zebrafish (Danio rerio). Methods The structure of zebrafish was observed by light microscopy and Micro-CT, and the images of its morphological structure were collected. The pictures of serial section (HE-stained) were corrected by Photoshop. The 3D model was established by the software of Autodesk 3ds Max. Results The results showed the zebrafish skeletal 3D imaging and serial section images (male: 144, female: 179). We realized the 3D reconstruction of the vital organs of zebrafish, and established the 3D anatomy interaction system zebrafish (V 1.0), which obtained the computer software copyright registration certificate (Certificate Number: soft word board the Di_0989966). Conclusion The zebrafish 3D anatomy interaction system software afforded the three-dimensional morphological basis for the study of zebrafish, and was helpful to histomorphology recognization for researchers.

Key words: zebrafish, Micro-CT, serial section, picture correction, three-dimension (3D) reconstruction