Evaluation of cardiomyocyte differentiation of CD73 subpopulations in adipose derived mesenchymal stem cells

Abstract

Abstract: Objective The cardiac differentiated potency about subpopulations of CD73+/CD73- ADMSCs were evaluated for selecting subsets of cardiac differentiation advantage. Methods Using flow cytometry to separate CD73 +/CD73- ADMSCs subgroups, their shape were detected by HE staining. After ADMSCs subgroups induced by 5-aza, the expression of c-TnT (myocardial specificity protein myocardial troponin) was detected by immunofluorescent chemical method, and using real-time PCR to check c-TnT and Gata-4 gene level changes. DAPI labeled subpopulations were myocardial transplanted, and then the expression of c-TnT were detected by immunofluorescence of transplanted cells. Results CD73+ subset were mainly small cells, possessed Stellate cells, triangle cells, slender and spindle cells. In contrast, CD73- cells showed a homogeneous morphologic pattern with wide flat or square shapes, dubbed big cells. After myocardium induction, the express rate of c-TnT is 45.5% in CD73+ ADMSCs , but in the CD73- subgroup is only 5.75%. CD73+ subsets was higher than negative subgroup in expression of c-TnT and Gata-4 gene (P < 0.01). CD73+ subsets was higher than negative subgroup in expression of c-TnT in vivo also. Conclusions The percentage of cardiomyocyte transdifferentiation in CD73+ ADMSCs was obviously higher than that in CD73- subset. The results could be demonstrated that CD73+ cell possessed preferable therapy effect for heart disease.

Key words: CD73, cTnT, Cardiomyocytes differentiation, Adipose-derived mesenchymal stem cells

GUO ZHIKUN Zhang Min Gao Jianhui Li Qiong Yu Xia Wang Jingjing. Evaluation of cardiomyocyte differentiation of CD73 subpopulations in adipose derived mesenchymal stem cells[J]. , 2016, 47(1): 49-54.

References

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