Effects of early-life stress on the development of the striatal neurons of mouse

doi:10.16098/j.issn.0529-1356.2016.04.003

Abstract

Abstract:

Objective To study the impact of early-life stress on the development of spiny neurons in the dorsal striatum. Methods The early-life stress animal model was created by changing the growth environment of new born mouse pups from postnatal day（P）2 to P 9 (P2-P9). The in situ hybridization, Golgi staining, and stereological analysis were employed to investigate the effect of stress on the soma, dendritic branches, and spines of striatal neurons. Results The striatal neurons in P9 C57BL/6J contained numerous dendritic branches and spines. Stress from P2 to P9 particularly affected the striatal neurons in the dorsolateral region, leading to abundant proximal dendritic branches (9.50±0.38 vs 6.50±0.23, n=6.8,P<0.05), and increased number of filopodia (8.15±0.51 vs 3.85±0.33 per 20 μm dendritic segment, n=6.8,P<0.05), but reduced dendritic spines (12.05±0.91 vs 20.02±0.73 per 20 μm dendritic segment, n=6.8,P<0.05). Conclusion Early-life stress interrupted the dendritic differentiation and postponed the maturation of spines of striatal neurons in the dorsolateral striatum.

Key words: Striatum, Early-life stress, Dendritic spine, Development, In situ hybridization, Mouse

XU Ben-ke MIAO Ying-ying SUN An-bang HE Yun LIU Yang CHEN Yun-cai. Effects of early-life stress on the development of the striatal neurons of mouse[J]. Acta Anatomica Sinica, 2016, 47(4): 449-455.

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