Differences of adipogentic differention characteristics between the mesenchymal stem cells from white adipose tissue and brown adipose tissue in rats

doi:10.16098/j.issn.0529-1356.2016.05.005

Abstract

Abstract:

Objective To study differences of adipogenic differentiation characteristics between the mesenchymal stem cells from white adipose tissue (WAT) and brown adipose tissue (BAT). Methods Fifteen male SD rats were used in this study, 3 for cell separation and 12 for cell transplantation. Adipose mesenchymal stem cells from WAT and BAT were separated and cultured in vitro. Oil red O staining was used to test the adipogenic differentiation rate of each kind of stem cells. Immunofluorescence techniques was used to test the adipogenic differentiation rate of each kind of stem cells, and to detect whether the cells were white fat cells or brown fat cells after adipogenic induction and differentiation. Both stem cells after 4’6-diamidino-2-phenylindole(DAPI) staining were transplanted to the groin area. Sample tissues were obtained in 1, 2 and 3 weeks. Immunofluorescence technique was used to detect the differentiation trend of the implanted cells. Results Proliferation rate of stem cells from WAT was significantly faster than that of BAT. Adipogenic differentiation rate of the former was 0.205±0.069, while the latter was 0.165±0.053. Adipogenic rates of the two kinds of stem cells had no statistical significance (P>0.05). After adipogenic cells were implanted in the body, brown adipose specific proteins uncoupling protein 1(UCP1) were expressed from two kinds of stem cells in 1, 2 and 3 weeks’ tissues. Conclusion Both stem cells of WAT and BAT cultured in vitro and in vivo were differentiated into brown fat cells after adipogentic induction.

Key words: Uncoupling protein 1, Brown adipose tissue, White adipose tissue, Adipose-derived mesenchymal stem cell, Adipogenic induction, Immunofluorescence, Rat

NIU Yan-fei ZHAO Guo-an CHANG Yu-qiao GAN Li YIN Guo-tian GUO Zhi-kun. Differences of adipogentic differention characteristics between the mesenchymal stem cells from white adipose tissue and brown adipose tissue in rats[J]. Acta Anatomica Sinica, 2016, 47(5): 607-613.

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