Effect of light intensity on expression of gonadotropin-releasing hormone in mesencephalon and diencephalon of Jinghong-Ⅰhens

doi:10.16098/j.issn.0529-1356.2019.03.004

Abstract

Abstract: Objcctive studies the effect of light intensity on expression of GnRH in Mesencephalon and Diencephalon of Jinghong-Ⅰhens. Method Jinghong-I hens aged 100d were randomly divided into four groups and applied a light of 30lx, 20lx, 10lx, and 1lx, respectively, to mensurate the distribution and expression of GnRH immunoreactive positive neurons in hen mesencephalon and diencephalon using immunohistochemical method. Results GnRH immunoreactive positive neurons were mainly observed in SGC, Imc, Ipc, SP/IPS, nbor, and SLu of mesencephalon. The mean gray values of positive neurons in 30lx and 20lx groups were significantly higher than those of 10lx and 1lx groups (p<0.05) in SGC, Imc, Ipc, and SLu. The percent cell area in SGC, Imc, and Ipc was the largest in 30 lx group, having no significant difference comparing 20lx, being significant higher than those of 10lx and 1lx (p<0.05). GnRH immunoreactive positive neurons were mainly observed in Rot, PHN, PVN, GLv, DLAm, CPa, and SM of diencephalon. The mean gray values of positive neurons in 30lx was the largest, being significantly higher than those of and 20lx, 10lx and 1lx groups (p<0.05) in Rot. There was no significant difference (p>0.05) between 30lx and 20lx group, but they were significant higher than those of 10lx and 1lx (p<0.05) in PHN, PVN, and GLv. The percent cell area in Rot was the largest in 30lx group, having no significant difference comparing 20lx, being significant higher than those of 10lx and 1lx (p<0.05). Those in 30lx and 20lx groups were significant higher than 10lx and 1lx (p<0.05) in PHN and PVN。Conclusion the expression of GnRH of 30lx and 20lx are higher significantly than 10lx and 1lx in hen mesencephalon and diencephalon.

LIU Hai-bin WUZhan-fu ZHANG Li-yong GUAN Li-hui CHEN Bao-jiang HU Man. Effect of light intensity on expression of gonadotropin-releasing hormone in mesencephalon and diencephalon of Jinghong-Ⅰhens[J]. Acta Anatomica Sinica, 2019, 50(3): 285-291.

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