Expression of elastin in rat cardiomyocytes and cardiomyocyte cell line H9C2

doi:10.16098/j.issn.0529-1356.2019.03.005

Abstract

Abstract:

Objective To detect the expression of elastin in cardiomyocytes and cardiomyocyte cell line H9C2. Methods The five fresh hearts of newborn and adult SD rats are made into frozen slices.Primary cardiomyocytes were isolated from the hearts of the twenty neonatal 3 days SD rats by the digestion of 0.05% trypsin and 0.075% type Ⅱ collagenase.The technology of immunochemical and immunofluorescence were used to detect the expression of elastin in rat myocardial tissue, primary cardiomyocytes and cardiomyocyte cell line H9C2.Elastin in supernatant of cultured cardiomyocyte cell line H9C2 was detected by ELISA. Results The co-expression of cardiac troponin T and elastin was found in rat cardiomyocytes, cardiomyocyte cell line H9C2,neonatal and adult SD rat myocardial tissues.The result of immunohistochemistry also revealed the expression of elastin in fibroblasts, smooth muscle cells, and interstitial substance.A small amount of elastin was detected in the supernatant of cardiomyocyte cell line H9C2. Conclusion Rat cardiomyocytes express elastin，it may be involved in the potential energy of myocardial cells and the construction of intercellular elastic fibers.

Key words: Elastin, Cardiomyocyte, Cardiomyocyte cell line H9C2, Immunohistochemistry, Immunofluorescence, Enzyme-linked immunosorbent assay, Rat

HAN Bei REN Ming-fen CHANG Yu-qiao GUO Zhi-kun. Expression of elastin in rat cardiomyocytes and cardiomyocyte cell line H9C2 [J]. Acta Anatomica Sinica, 2019, 50(3): 292-296.

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