Objective  To study the distribution characteristics of single nucleotide polymorphisms (SNP) of miR-107 gene rs2296616 C/T in Guangxi healthy population and comparison with that in different ethnic populations, and further to explore the correlation between rs2296616 C/T SNP and blood lipid level.   Methods  The polymorphisms of miR-107 gene rs2296616C/T among 372 Chinese healthy individuals of Guangxi were detected by multiplex SNaPshot and DNA sequencing method , and the blood lipidrelated indexes were detected by 7600 biochemical analyzer. The distribution of rs2296616 C/T polymorphism among different ethnic groups and the differences of blood lipid levels among different genotypes were compared by statistical method .   Results  MiR-107 gene rs2296616 C/T SNP contained TT（9.1%）, CT（8.9%）genotypes and T（95.6%）, C（4.4%）alleles in Guangxi healthy population. The frequencies of genotype and allele distribution of rs2296616 C/T were not significantly different among genders in Guangxi population（P>0.05）. However, there were significant differences in the genotype and allele frequency of miR-107 gene rs2296616 C/T in Guangxi healthy population compared with those of Europeans, Japanese, Africans, Mexicans and Indians published in HapMap(P<0.05), no significant difference compared with HapMap-HGB(P>0.05). When compared the blood lipid level among two genotypes in rs2296616 C/T, we found that the level of high density lipoprotein cholesterol(HDL-C) with TT genotype was significantly different from that of CT group(P<0.05).   Conclusion  There are different degrees of variation in the polymorphisms of rs2296616 C/T of miR-107 gene between Guangxi people and other ethnic populations. The polymorphism of rs2296616 C/T locus is related to the level of HDL-C.