Expression and clinical significance of CC motif chemokine ligand 20 in esophageal carcinoma based on the Cancer Genome Atlas data mining

doi:10.16098/j.issn.0529-1356.2022.01.008

Abstract

Abstract:

Objective To explore the expression and significance of CC mokif chemokine ligand 20(CCL20) gene in esophageal carcinoma and its influence on the progression and prognosis of the disease. Methods The expression data of esophageal cancer were obtained from the Cancer Genome Atlas (TCGA) database, and were collected from cBioPortal(www.cbioportal.org).The survival curve was analyzed by Kaplan-Meier method . Through the website (http://ualcan.path.uab.edu) the objective was to analyze the expression of CCL20 gene in esophageal cancer tissues, the relationship between CCL20 and obesity and the methylation level of CCL20 in esophageal cancer. cBioPortal was used to analyze the copy number and mRNA expression of CCL20 gene. Finally, the expression differences of CCL20 in esophageal cancer tissues and adjacent tissues were analyzed by mRNA and protein levels. Results CCL20 was highly expressed in esophageal cancer, especially adenocarcinoma. The survival rate of patients with high expression of CCL20 was reduced. The expression of CCL20 was directly proportional to the degree of obesity in patients with esophageal cancer and inversely proportional to the degree of methylation. The expression of CCL20 in esophageal cancer tissues increased significantly. Conclusion CCL20 gene expression level is associated with poor prognosis of esophageal cancer patients.

Key words:

Esophageal cancer, CC motif chemokine ligand 20, The Cancer Genome Atlas database, Prognosis, Immunohistochemistry, Human

CLC Number:

R735.1

QIN Bo WANG Zheng-yang YIN Fan-xiang YAN Dan ZHU Zhu. Expression and clinical significance of CC motif chemokine ligand 20 in esophageal carcinoma based on the Cancer Genome Atlas data mining[J]. Acta Anatomica Sinica, 2022, 53(1): 60-65.

References

［1］ Torre LA, Bray F, Siegel RL, et al. Global cancer statistics［J］.CA Cancer J Clin, 2012，65 (2):7-108.

［2］ Lordick F, Hlscher AH, Haustermans K, et al. Multimodal treatment of esophageal cancer［J］. Langenbecks Arch Surg, 2013, 398 (2):177-187.

［3］ Chen ZhJ, Liao ShY, Wu WL, et al. Expression of chemokines CCL20 and SDF-1 in colorectal cancer and their relationship with lymph node and liver metastasis［J］. Guangdong Medicine, 2018, 39(17):2598-2601，2605. (in Chinese)

陈志京, 廖山婴, 吴伍林, 等. 趋化因子CCL20、SDF-1在大肠癌中的表达及其与淋巴结及肝脏转移的关系［J］. 广东医学, 2018, 39(17):2598-2601, 2605.

［4］ Walch-Rückheim B, Mavrova R, Henning M, et al. Stromal Fibroblasts Induce CCL20 through IL6/C/EBPβ to support the recruitment of Th17 cells during cervical cancer progression［J］. Cancer Res, 2015,75(24):5248-5259.

［5］ Geismann C, Grohmann F, Dreher A, et al. Role of CCL20 mediated immune cell recruitment in NF-κB mediated TRAIL resistance of pancreatic cancer［J］. Biochim Biophys Acta Mol Cell Res, 2017, 1864(5):782-796.

［6］ Kadomoto S, Izumi K, Mizokami A. The CCL20-CCR6 axis in cancer progression ［J］. Int J Mol Sci, 2020, 21(15):5186.

［7］ Lee AYS, K?rner H. The CCR6-CCL20 axis in humoral immunity and T-B cell immunobiology［J］. Immunobiology, 2019, 224(3):449-454.

［8］ Xiao JL, Zheng X, Deng H, et al. Expression of CCR6 in esophagealsquamous cell carcinoma and its effects on epithelial-to-mesenchymal transition［J］. Oncotarget, 2017, 8(70): 115244-115253.

［9］ Lian J, Liu S, Yue Y, et al. Eomes promotes esophageal carcinoma progression by recruiting Treg cells through the CCL20-CCR6 pathway［J］. Cancer Sci, 2021, 112(1):144-154.

［10］ Wang Y, Lyu Zh, Qin Y, et al. FOXO1 promotes tumor progression by increased M2 macrophage infiltration in esophageal squamous cell carcinoma［J］. Theranostics, 2020,10(25):11535-11548.

［11］ Liu JY, Li F, Wang LP, et al. CTL-vs Treg lymphocyte-attracting chemokines, CCL4 and CCL20, are strong reciprocal predictive markers for survival of patients with oesophageal squamous cell carcinoma［J］. Br J Cancer, 2015,113(5): 747-755.

［12］ Lauby-Secretan B, Scoccianti Ch, Loomis D, et al. Body fatness and cancer——viewpoint of the IARC Working Group［J］. N Engl J Med, 2016, 375: 794-798.

［13］ Wunderlich CM, Ackermann PJ, Ostermann AL, et al. Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment［J］. Nat Commun, 2018, 9(1):1646.

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